公开(公告)号：US20100209932A1

公开(公告)日：2010-08-19

申请号：US12706627

申请日：2010-02-16

申请人： EUGENE G. SPIER

发明人： EUGENE G. SPIER

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6809 ,  C12Q1/6865 ,  C12Q2525/301 ,  C12Q2525/143 ,  C12Q2525/207 ,  C12Q2565/501

摘要： The present teachings provide methods for reverse transcribing, and detecting, a plurality of small nucleic acids such as micro RNAs, from the same reaction mixture as a plurality of messenger RNAs. High levels of multiplexing are provided by the use of a plurality of zip-coded stem-loop reverse transcription primers, along with an oligo-dT-promoter-containing reverse transcription primer, in the same reverse transcription reaction mixture. The resulting products can be amplified in an in vitro transcription reaction, and detected on a solid support such as an array. The present teachings also provide compositions, kits, and devices for performing and detecting the reverse transcription reactions described herein.

摘要翻译： 本教导提供了与多个信使RNA相同的反应混合物逆转录和检测多个小核酸如微RNA的方法。 通过在相同的逆转录反应混合物中使用多个zip编码的茎环逆转录引物以及含有含有dT启动子的逆转录引物提供高水平的复用。 所得产物可以在体外转录反应中扩增，并在固体支持物如阵列上检测。 本教导还提供用于进行和检测本文所述的逆转录反应的组合物，试剂盒和装置。