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公开(公告)号：US20230324373A1
公开(公告)日：2023-10-12
申请号：US17904505
申请日：2021-02-09
Inventor： Yi Yang , Yuzheng Zhao , Xie Li , Zeyi Zhang , Xiuze Zhang , Li Huang
IPC: G01N33/53 , G01N33/533 , C07K14/245
CPC classification number: G01N33/5308 , G01N33/533 , C07K14/245
Abstract: Disclosed in the present invention are a pyruvic acid optical probe, a preparation method therefor, and an application thereof. One aspect of the present invention is the disclosure of an optical probe, which includes a pyruvic acid-sensitive polypeptide and an optically active polypeptide, wherein the optically active polypeptide is located within the sequence of the pyruvic acid-sensitive polypeptide. The present invention also discloses a preparation method for the probe and an application of said probe in pyruvic acid measurement.
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公开(公告)号：US20230203506A1
公开(公告)日：2023-06-29
申请号：US17787803
申请日：2020-12-21
Inventor： Yi Yang , Yuzheng Zhao , Xie Li , Xiuze Zhang , Li Huang
IPC: C12N15/62 , C07K14/245 , C12N15/63 , G01N33/58
CPC classification number: C12N15/62 , C07K14/245 , C12N15/63 , G01N33/582 , C07K2319/60 , C12N2320/10
Abstract: An optical probe, comprising a lactic acid-sensitive polypeptide or a functional variant thereof and an optically active polypeptide or a functional variant thereof; the optically active polypeptide or the functional variant thereof is located in the sequence of the lactic acid-sensitive polypeptide or the functional variant thereof. A preparation method for the described probe and an application thereof in the detection of lactic acid.
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公开(公告)号：US20230296617A1
公开(公告)日：2023-09-21
申请号：US17904503
申请日：2021-02-09
Inventor： Yi Yang , Yuzheng Zhao , Chenxia Zhang , Li Huang , Yejun Zou , Xie Li , Shuning Liu
CPC classification number: G01N33/6812 , G01N33/582 , C12N15/63
Abstract: Disclosed is a tryptophan optical probe, a preparation method therefor and the use thereof. Disclosed is an optical probe, comprising a tryptophan-sensitive polypeptide or a functional variant thereof and an optically active polypeptide or a functional variant thereof, wherein the optically active polypeptide or the functional variant thereof is located in the sequence of the tryptophan-sensitive polypeptide or the functional variant thereof. Furthermore, disclosed are a method for preparing the above-mentioned probe and the use thereof in the detection of tryptophan.
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公开(公告)号：US20230296616A1
公开(公告)日：2023-09-21
申请号：US17904498
申请日：2021-02-09
IPC: G01N33/68 , G01N33/533 , C07K14/00
CPC classification number: G01N33/6812 , G01N33/533 , C07K14/001
Abstract: Provided is an arginine fluorescent probe, comprising a polypeptide B that responds to arginine and a fluorescent protein A that expresses arginine; the fluorescent protein A is inserted into the polypeptide B, B is divided into an upper structural part and a lower structural part, B1 and B2, and a probe structure represented by the formula B1-A-B2 is formed; optimized mutants are likewise obtained by truncation and site-directed mutagenesis at different positions, and specific binding of the polypeptide B and arginine leads to a change in the fluorescence signal of the fluorescent protein A; and the polypeptide B is an arginine binding protein or a mutant thereof. The arginine fluorescent probe provided by the present invention has a relatively small protein molecular weight, is easy to express, experiences large dynamic changes in fluorescence, has good specificity, can be expressed, by means of genetic manipulation, in different subcellular organelles of cells, and can be used for the high-throughput, quantitative detection of arginine inside and outside of cells.
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