公开(公告)号：US20240352087A1

公开(公告)日：2024-10-24

申请号：US18579142

申请日：2022-07-14

Applicant: Emory University

Inventor： Rafi Ahmed ,  Andreas Wieland ,  Christiane Eberhardt

IPC: C07K14/725

CPC classification number: C07K14/7051 ,  C07K2319/60

Abstract: This disclosure relates T cell receptors (TCR) having antigenic specificity for an epitopes of human papillomavirus (HPV) proteins such as E2, E5, and E6. Related poly peptides and proteins, as well as related nucleic acids, recombinant expression vectors, host cells, and populations of cells are also provided. Pharmaceutical compositions relating to the TCRs are also provided. Also disclosed are methods of detecting the presence of a condition in a mammal and methods of treating or preventing a condition in a mammal, wherein the condition is an epithelial cancer. HPV infection, or HPV-positive premalignancy.

公开(公告)号：US20240343833A1

公开(公告)日：2024-10-17

申请号：US18752484

申请日：2024-06-24

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Mohamed Yosry Hassan Mohamed ,  Tobias Oelschlaegel

IPC: C07K16/40 ,  G01N33/68

CPC classification number: C07K16/40 ,  C12Y203/02013 ,  G01N33/6854 ,  C07K2317/20 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/622 ,  C07K2319/60

Abstract: The present invention relates to a modified antibody comprising a heavy chain and a light chain, wherein the antibody is modified to include in one or more of its immunoglobulin polypeptide chains one or more first recognition site(s) for the transglutaminase from Kutzneria albida (KalbTG) or a functionally active variant thereof. The one or more first recognition site(s) are introduced at one or more selected position(s) within an antibody's heavy chain and/or an antibody's light chain. The invention further relates to one or more nucleic acid(s) encoding an immunoglobulin polypeptide chain including the one or more recognition site(s), a site-specifically conjugated antibody comprising the modified antibody and one or more labelling domain(s) covalently attached to one or more first recognition sites, a kit for producing the conjugated antibody, a method of specifically labelling the modified antibody by way of site-specific conjugation, the use of the modified antibody for producing a specifically site-specifically conjugated antibody, a method of detecting a target in a sample and the use of the site-specifically conjugated antibody in the detection of a target and/or in the diagnosis.

公开(公告)号：US12105091B2

公开(公告)日：2024-10-01

申请号：US16761786

申请日：2018-11-06

Applicant: VIB VZW ,  Universiteit Gent

Inventor： An Hendrix ,  Olivier De Wever ,  Edward Geeurickx ,  Joke Vandesompele ,  Pieter Mestdagh ,  Sven Eyckerman

IPC: G01N33/58 ,  C07K14/16 ,  C07K14/435 ,  C12Q1/6876 ,  G01N21/64 ,  G01N33/53

CPC classification number: G01N33/582 ,  C07K14/161 ,  C07K14/43595 ,  C12Q1/6876 ,  G01N21/6428 ,  G01N33/5308 ,  C07K2319/60 ,  C12Q2600/158 ,  G01N2021/6439 ,  G01N2496/30

Abstract: This disclosure relates to the field of research and clinical usage of extracellular vesicles. More in particular, this disclosure relates to usages of recombinant extracellular vesicles comprising a) a self-assembling protein that directs its own release through vesicles as a luminal membrane-bound protein and b) a heterologous marker. Indeed, the disclosure provides recombinant extracellular vesicles that can be used as a biological reference material in methods to quantify extracellular vesicles in a sample, and/or, that can be used to calibrate a device for sample extracellular vesicles analysis, and/or, that can be used to evaluate the isolation process of extracellular vesicles. This disclosure thus relates to improving the accuracy of an extracellular vesicle-based diagnosis.

公开(公告)号：US20240318151A1

公开(公告)日：2024-09-26

申请号：US18609655

申请日：2024-03-19

Applicant: Qatar Foundation for Education, Science and Community Development

Inventor： Kabir Hassan Biswas

IPC: C12N9/02

CPC classification number: C12N9/0069 ,  C07K2319/60 ,  C12Y113/12005

Abstract: The instant disclosure relates to methods for luciferase modification.

公开(公告)号：US20240287140A1

公开(公告)日：2024-08-29

申请号：US18415225

申请日：2024-01-17

Applicant: Sunstate Biosciences, LLC

Inventor： Changjun Yu

IPC: C07K14/00 ,  C07K14/765

CPC classification number: C07K14/003 ,  C07K14/765 ,  C07K2319/60

Abstract: The invention provides compositions comprising a single protein having one or more biopolymers or modified biopolymers for therapeutics and diagnostics tightly bound therein. The compositions are useful to enhance efficacy and water solution of biopolymers, decrease their toxicity and/or to widen therapeutic window of the biopolymers. The invention also provides methods for preparing such compositions.

公开(公告)号：US20240269286A1

公开(公告)日：2024-08-15

申请号：US18454275

申请日：2023-08-23

Applicant: EOS Neuroscience, Inc.

Inventor： Alan Horsager ,  Kenneth Greenberg ,  Benjamin C. Matteo ,  Edward S. Boyden ,  Douglas G. Ririe ,  James C. Eisenach ,  Christian Wentz

IPC: A61K41/00 ,  A61K31/7088 ,  A61N5/06 ,  C07K14/195 ,  C07K14/215 ,  C07K14/37

CPC classification number: A61K41/00 ,  A61K31/7088 ,  A61N5/062 ,  C07K14/195 ,  C07K14/215 ,  C07K14/37 ,  C07K2319/60 ,  C12N2750/14143 ,  C12N2810/6027

Abstract: Provided are compositions and methods for the selective silencing of neurons in pain pathway by using a combination of inhibitory light-sensitive protein gene transfer and wavelength specific illumination.

公开(公告)号：US20240190928A1

公开(公告)日：2024-06-13

申请号：US18519014

申请日：2023-11-26

Applicant: DWI-Leibniz-Institut Fur Interaktive Materialien E.V.

Inventor： Ulrich SCHWANEBERG ,  Nursen Sözer ,  Alan Mertens ,  Mehdi Davari Dolatabadi ,  Felix Jakob ,  Shohana Islam ,  Lina Apitius

IPC: C07K14/435 ,  C07K14/31 ,  C12Q1/42 ,  G01N33/44

CPC classification number: C07K14/43563 ,  C07K14/31 ,  C07K14/43509 ,  C12Q1/42 ,  G01N33/442 ,  C07K2319/60

Abstract: The present invention pertains to a novel fusion protein and/or fusion peptide, preferably for use in the separation from and/or detection in an environment of one or more target polymers or plastics, e.g., one or more target polymer fragments and/or particles or target plastic fragments and/or particles, preferably wherein the one or more target polymer particles or target plastic particles are microplastics; a method of preparing such novel fusion protein and/or fusion peptide, a system and kit comprising the novel fusion protein and/or fusion peptide and a (polymer or non-polymer) carrier or carrier system, a use of the novel fusion protein and/or fusion peptide or of a system and kit as mentioned in the separation from and/or detection in an environment of one or more target polymers or plastics, e.g., one or more target polymer fragments and/or particles or target plastic fragments and/or particles, preferably wherein the one or more target polymer particles or target plastic particles are microplastics; a method of separation of one or more target polymers or plastics from an environment, e.g., one or more target polymer fragments and/or particles or target plastic fragments and/or particles, and a method of detection of one or more target polymers or plastics in an environment, e.g., one or more target polymer fragments and/or particles or target plastic fragments and/or particles, preferably wherein the one or more target polymer particles or target plastic particles are microplastics.

公开(公告)号：US20240175865A1

公开(公告)日：2024-05-30

申请号：US17772238

申请日：2021-01-30

Applicant: Hans-Joachim WIEDEN ,  Dustin D. SMITH ,  Dylan GIRODAT ,  Luc Arden Hamilton ROBERTS ,  Her Majesty The Queen in Right of Canada, as represented by the Minister of Agriculture and Agri-Foo

Inventor： Hans-Joachim WIEDEN ,  Dustin D. SMITH ,  Dylan GIRODAT ,  Luc Arden Hamilton ROBERTS ,  D. Wade ABBOT

IPC: G01N33/53 ,  C07K14/24 ,  C07K14/245 ,  C07K14/315 ,  G01N33/533 ,  G01N33/542 ,  G16B15/30

CPC classification number: G01N33/5308 ,  C07K14/24 ,  C07K14/245 ,  C07K14/3156 ,  G01N33/533 ,  G01N33/542 ,  G16B15/30 ,  C07K2319/60 ,  G01N2333/24 ,  G01N2333/3156

Abstract: Improved processes for rationally-designing and producing biomolecules are disclosed herein. More specifically, disclosed processes comprise the steps of (i) selecting at least one biomolecule suitable for modification; (ii) obtaining at least one structure of the at least one biomolecule; (iii) simulating the molecular dynamics of the at least one structure to generate dynamic information about at least one position within the at least one structure; (iv) using the dynamic information to calculate a score for the at least one position; (v) comparing the score with at least one reference score to identify at least one target position within the biomolecule suitable for modification; and (vi) modifying the at least one target position. As proof-of-concept, the disclosed processes were used to rationally-design and produce maltooligosaccharide-detecting biosensors based on Streptococcus pneumoniae MalX, homogalacturonan breakdown product-detecting biosensors based on Yersinia enterocolitica TogB, and biosensors for observing conformational changes based on Escherichia coli EF-Tu.

公开(公告)号：US11987835B2

公开(公告)日：2024-05-21

申请号：US18067977

申请日：2022-12-19

Applicant: University of Oregon

Inventor： Catherine Robinson ,  Emily Sweeney ,  Karen Guillemin

IPC: C12Q1/48 ,  A61K35/74 ,  C07K14/195 ,  C12N9/12 ,  G01N33/68

CPC classification number: C12Q1/485 ,  A61K35/74 ,  C07K14/195 ,  C12N9/1241 ,  C12Y207/07065 ,  G01N33/6806 ,  C07K2319/60 ,  G01N2333/9125

Abstract: Provided are SpdE polypeptides and variants and nucleic acids encoding the SpdE polypeptides and variants. Also provided are vectors including one or more nucleic acids encoding a SpdE polypeptide or variant and cells including a nucleic acid encoding the SpdE polypeptide or variant, as well as cells expressing a SpdE polypeptide or variant and compositions including such cells and a pharmaceutically acceptable carrier. Finally, methods of detecting presence and/or amount of one or more amino acids in a sample are provided. The methods include contacting the sample with a SpdE protein, measuring diguanylate cyclase activity of the SpdE protein; and comparing the diguanylate cyclase activity of the SpdE protein to a control. The methods can utilize isolated SpdE protein or a cell expressing a SpdE protein.

公开(公告)号：US20240156926A1

公开(公告)日：2024-05-16

申请号：US18302645

申请日：2023-04-18

Applicant: UCL Business Ltd

Inventor： Martin Pulé ,  Brian Philip

IPC: A61K39/00 ,  A61K35/17 ,  C07K14/705 ,  C07K14/725 ,  C07K16/28 ,  C12N15/85 ,  G01N33/50

CPC classification number: A61K39/0011 ,  A61K35/17 ,  C07K14/70503 ,  C07K14/7051 ,  C07K14/70517 ,  C07K14/70596 ,  C07K16/2887 ,  C07K16/2896 ,  C12N15/85 ,  G01N33/5005 ,  A61K48/00 ,  C07K2317/34 ,  C07K2317/52 ,  C07K2319/03 ,  C07K2319/40 ,  C07K2319/60 ,  C07K2319/70

Abstract: The present invention provides a polypeptide having the formula: St—R1—S1—Q—S2—R2 wherein St is a stalk sequence which, when the polypeptide is expressed at the surface of a target cell, causes the R and Q epitopes to be projected from the cell surface; R1 and R2 are a Rituximab-binding epitopes each having the an amino acid sequence selected from the group consisting of SEQ ID No. 1, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 and 16 or a variant thereof which retains Rituximab-binding activity; S1 and S2 are optional spacer sequences, which may be the same or different; and Q is a QBEnd10-binding epitope having the amino acid sequence shown as SEQ ID No. 2 or a variant thereof which QBEnd10-binding activity. The invention also provides a nucleic acid sequence encoding such a polypeptide and uses thereof in adoptive cell transfer.