公开(公告)号：US06821778B1

公开(公告)日：2004-11-23

申请号：US08610195

申请日：1996-02-28

申请人： Edgar G. Engleman ,  Anita Mehta ,  Masaru Takamizawa ,  Francesco Fagnoni ,  Sergiusz Markowicz

发明人： Edgar G. Engleman ,  Anita Mehta ,  Masaru Takamizawa ,  Francesco Fagnoni ,  Sergiusz Markowicz

IPC分类号： C12N508

CPC分类号： C12N5/0636 ,  A61K2039/5158 ,  A61K2039/57

摘要： This invention relates to methods of using human dendritic cells to present antigens for the induction of antigen-specific T cell-mediated immune responses. In particular, it relates to the isolation of dendritic cells from human blood, exposing the cells to antigens, co-culturing the antigen-pulsed dendritic cells with &ggr;&dgr;-T cell receptor-positive-T cells (&ggr;&dgr;-TCR+ T cells) obtained from unprimed or weakly primed individuals for the stimulation of antigen-specific T cell proliferative and cytotoxic activities. The dendritic cell antigen presentation system described herein has a wide range of applications, including but not limited to, activation and expansion of large numbers of antigen-specific major histocompatibility complex-unrestricted T cells for use in adoptive cellular immunotherapy against infectious diseases and cancer.

摘要翻译： 本发明涉及使用人树突状细胞呈递抗原以诱导抗原特异性T细胞介导的免疫应答的方法。 特别地，它涉及从人血液中分离树突状细胞，将细胞暴露于抗原，用gammadelta-T细胞受体阳性T细胞（gammadelta-TCR + T细胞）共同培养抗原脉冲的树突状细胞 ）从未引导或弱引发的个体获得，用于刺激抗原特异性T细胞增殖和细胞毒活性。 本文描述的树突状细胞抗原呈递系统具有广泛的应用，包括但不限于大量抗原特异性主要组织相容性复合物无限制T细胞的活化和扩增，用于针对传染病和癌症的过继细胞免疫治疗。