公开(公告)号：US20060073582A1

公开(公告)日：2006-04-06

申请号：US11154203

申请日：2005-06-16

申请人： Edward Meehan ,  Liqing Chen ,  Enrico DiGiammarino ,  Thomas Hodge

发明人： Edward Meehan ,  Liqing Chen ,  Enrico DiGiammarino ,  Thomas Hodge

IPC分类号： G01N33/48 ,  C12N9/12 ,  G06F19/00

CPC分类号： C12N9/16 ,  C07K2299/00 ,  G01N2500/04

摘要： The present invention relates to crystalline Rab9 in complex with either GDP or the GTP analog Guanosine 5′-(β,γ-imido) triphosphate (GppNHp), the three dimensional coordinates and structures of Rab9 in Rab9-GDP and Rab9-GppNHp complexes, and uses thereof for drug design. In particular, the present invention relates to methods for producing Rab9 crystals of sufficient quality to obtain a determination of the three dimensional structure of Rab9 to a high resolution in both its GTP-bound (on/active) and GDP-bound (off/inactive) conformations. The present invention also relates to a computer-readable medium encoded with the three dimensional coordinates of Rab9 in Rab9-GDP and Rab9-GppNHp complexes wherein, using a graphical display software program, the three dimensional coordinates create an electronic file that can be visualized on a computer capable of representing the electronic file as a three dimensional image.

摘要翻译： 本发明涉及与GDP或GTP类似物鸟苷5' - （β，γ-亚氨基）三磷酸（GppNHp）复合的结晶Rab9，Rab9-GDP和Rab9-GppNHp复合物中Rab9的三维坐标和结构， 并将其用于药物设计。 特别地，本发明涉及用于生产足够质量的Rab9晶体的方法，以在其GTP结合（开/主动）和GDP-结合（关/非活性）两者中获得Rab9的三维结构确定为高分辨率 ）构象。 本发明还涉及用Rab9-GDP和Rab9-GppNHp复合物中的Rab9的三维坐标编码的计算机可读介质，其中使用图形显示软件程序，三维坐标创建可以在 能够将电子文件表示为三维图像的计算机。

公开(公告)号：US20070134743A1

公开(公告)日：2007-06-14

申请号：US11302975

申请日：2005-12-14

申请人： Erica Phillips ,  Enrico DiGiammarino ,  Kevin McGrath

发明人： Erica Phillips ,  Enrico DiGiammarino ,  Kevin McGrath

IPC分类号： G01N33/574 ,  G01N33/569 ,  C12M3/00

CPC分类号： G01N33/56961 ,  C12Q1/37 ,  G01N33/558

摘要： Methods and devices for the detection of proteins secreted by the hyphal growth form of Candida species are disclosed. The disclosed devices may constitute a method for the diagnosis of acute or chronic infections, including candidiasis, caused by microorganisms of the species Candida, such as C. albicans, for example. The devices of the present invention incorporate antibodies specific to secreted aspartyl protease proteins whose expression is upregulated upon the conversion of the Candida species from the commensal to the pathogenic form. The antibodies may be used in assays to allow the diagnosis of candidal infections and disease conditions. Either monoclonal antibodies or polyclonal antibodies may be used, and in the case of the monoclonals, the specific epitopes of the SAP protein may be detected as well as the SAP protein itself.

摘要翻译： 公开了用于检测由假丝酵母属菌丝生长形式分泌的蛋白质的方法和装置。 所公开的装置可以构成用于诊断例如由假丝酵母属物种的微生物引起的急性或慢性感染（包括念珠菌病）的方法，例如白色念珠菌。 本发明的装置包含对分泌的天冬氨酰蛋白酶蛋白特异性的抗体，其在将假丝酵母菌种从共生转化为致病形式时，其表达被上调。 抗体可用于测定以允许诊断念珠菌感染和疾病状况。 可以使用单克隆抗体或多克隆抗体，并且在单克隆的情况下，可以检测SAP蛋白的特异性表位以及SAP蛋白本身。

公开(公告)号：US20070134747A1

公开(公告)日：2007-06-14

申请号：US11302991

申请日：2005-12-14

申请人： Enrico DiGiammarino ,  Kevin McGrath

发明人： Enrico DiGiammarino ,  Kevin McGrath

IPC分类号： G01N33/53

CPC分类号： G01N33/573 ,  G01N2333/40 ,  G01N2333/918

摘要： Methods and devices for the detection of proteins secreted by the pathogenic growth form of Candida species are disclosed. The disclosed devices may constitute a method for the diagnosis of acute or chronic infections, including candidiasis, caused by microorganisms of the Candida species, such as C. albicans, for example. The devices of the present invention incorporate antibodies specific to lipase proteins whose expression is differentiated upon the conversion of the Candida species from the commensal to the pathogenic form. The antibodies may be used in assays to allow the diagnosis of candidal infections and disease conditions. Either monoclonal antibodies or polyclonal antibodies may be used, and in the case of the monoclonals, the specific epitopes of the LIP protein may be detected as well as the LIP protein itself.

摘要翻译： 公开了用于检测由假丝酵母属病原体生长形式分泌的蛋白质的方法和装置。 所公开的装置可以构成用于诊断例如由假丝酵母属物种的微生物引起的急性或慢性感染（包括念珠菌病）的方法，例如白色念珠菌。 本发明的装置包含对脂肪酶蛋白特异性的抗体，其表达在将念珠菌属物种从共生转化为致病形式时是分化的。 抗体可用于测定以允许诊断念珠菌感染和疾病状况。 可以使用单克隆抗体或多克隆抗体，并且在单克隆抗体的情况下，可以检测LIP蛋白的特异性表位以及LIP蛋白本身。