公开(公告)号：US20060051756A1

公开(公告)日：2006-03-09

申请号：US10512219

申请日：2003-04-22

申请人： Eiichi Tamiya ,  Masaaki Kobayyashi ,  Ichiro Nagashio

发明人： Eiichi Tamiya ,  Masaaki Kobayyashi ,  Ichiro Nagashio

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： B01L3/502 ,  B01L3/50825 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/087 ,  B01L2400/0683

摘要： A gene detection tool comprising a sealable reaction chamber and a sealable detection chamber and a partition member positioned between the reaction chamber and the detection chamber that separates the reaction chamber and the detection chamber. The partition member is capable of unsealing; the reaction chamber is comprised of at least two members capable of forming a sealed reaction chamber by mutually fitting an opening means provided therein; and the detection chamber or reaction chamber comprises a gene detection element. A gene detection method in which a gene amplification operation and a gene detection operation are conducted in a sealed state using a gene detection device comprising a sealable reaction chamber and a sealable detection chamber, having a partition member which is positioned between the reaction chamber and the detection chamber and which separates the reaction chamber from the detection chamber, with test sample and gene amplification reaction solution being packed in said reaction chamber and a gene-binding agent capable of binding with a gene and generating a detectable signal being packed in said detection chamber. (1) At least the reaction chamber is imparted with conditions capable of amplifying the gene to be detected; (2) following the operation of (1), the partition member is unsealed while maintaining the gene detection tool in a sealed state; (3) through a passage created in the partition member by the unsealing, the solution in the reaction chamber is brought into contact with the gene-binding agent capable of binding the gene in the detection chamber and generating a detectable signal; and (4) the signal obtained is detected. A gene detection kit comprising the gene detection tool, a gene amplification reaction solution or gene amplification reagent, and a gene-binding agent capable of binding a gene and generating a detectable signal.

摘要翻译： 一种基因检测工具，其包括可密封的反应室和可密封的检测室以及位于所述反应室和所述检测室之间的分隔部件，所述分隔部件分离所述反应室和所述检测室。 分隔构件能够开封; 反应室由至少两个能够通过相互配合设置在其中的开启装置形成密封反应室的构件组成; 并且检测室或反应室包括基因检测元件。 一种基因检测方法，其中使用包括可密封反应室和可密封检测室的基因检测装置在密封状态下进行基因扩增操作和基因检测操作，所述基因检测装置具有位于反应室和反应室之间的分隔部件 检测室，并且将反应室与检测室分离，将测试样品和基因扩增反应溶液包装在所述反应室中，以及能够与基因结合并产生可检测信号的基因结合剂，所述基因结合剂包装在所述检测室 。 （1）至少使反应室具有能够扩增待检测基因的条件; （2）在（1）的操作之后，分隔构件在将基因检测工具保持在密封状态的同时被开封; （3）通过开封在分隔构件中产生的通道，使反应室中的溶液与能够结合检测室中的基因并产生可检测信号的基因结合剂接触; 和（4）检测所获得的信号。 一种基因检测试剂盒，其包含基因检测工具，基因扩增反应溶液或基因扩增试剂，以及能够结合基因并产生可检测信号的基因结合剂。