公开(公告)号：US20090159458A1

公开(公告)日：2009-06-25

申请号：US12226060

申请日：2007-03-29

申请人： Eiichi Tamiya ,  Naoki Nagatani ,  Teruko Yuhi ,  Kagan Kerman ,  Koutarou Idegami ,  Miyuki Chikae

发明人： Eiichi Tamiya ,  Naoki Nagatani ,  Teruko Yuhi ,  Kagan Kerman ,  Koutarou Idegami ,  Miyuki Chikae

IPC分类号： G01N27/416 ,  G01N33/543

CPC分类号： G01N33/5438

摘要： [Problems] A highly sensitive and accurate determination of a test substance is achieved without the need of complicated procedures.[Means for solving the Problems] The presence or absence or the concentration of a test substance 3 can be determined by gathering around the surface of a working electrode 1 metal microparticles 5 in an amount corresponding to the amount of the test substance 3 contained in a test solution, oxidizing the metal microparticles 5 electrochemically, measuring the value of a current produced by electrochemically reducing the oxidized metal, and determining the presence or absence or the concentration of the substance 3 based on the current value. It is preferred that the electrochemical oxidation of the metal microparticles 5 be conducted while maintaining the potential of the working electrode 1 at a value equal to a potential employed for the electrochemical oxidization of the metal microparticles 5.

摘要翻译： [问题]在不需要复杂程序的情况下实现对测试物质的高度灵敏和准确的测定。 [解决问题的方法]测试物质3的存在或不存在或浓度可以通过将工作电极1的金属微粒5的表面聚集在与金属微粒5中包含的测试物质3的量相当的量 测试溶液，电化学氧化金属微粒5，测量通过电化学还原氧化金属产生的电流的值，并根据当前值确定物质3的存在或不存在或浓度。 优选的是，在将工作电极1的电位保持在与金属微粒5的电化学氧化的电位相等的值的情况下，进行金属微粒5的电化学氧化。

公开(公告)号：US08927219B2

公开(公告)日：2015-01-06

申请号：US12744064

申请日：2008-11-25

申请人： Koutarou Idegami ,  Takashi Yoneda ,  Yuzuru Takamura

发明人： Koutarou Idegami ,  Takashi Yoneda ,  Yuzuru Takamura

IPC分类号： G01N33/53 ,  G01N33/74 ,  G01N33/558 ,  G01N33/94

CPC分类号： G01N33/54366 ,  G01N33/558 ,  G01N33/9493

摘要： A method for evaluating the quality of a blood sample, comprising the steps of: mixing labeled anti-cortisol antibodies with a blood sample to be subjected, to conduct the antigen-antibody reaction of the labeled anti-cortisol antibody with cortisol contained in the blood sample, developing a mixture obtained in the above step on an immunochromatographic test strip having a substrate on which cortisol is immobilized to cause the antigen-antibody reaction of the labeled anti-cortisol antibodies which are free in the mixture with the cortisol immobilized on the substrate, thereby bonding the antibody to the cortisol, determining the amount of the labeled anti-cortisol antibodies bonded to the cortisol in the above step, and evaluating whether or not the blood sample has a quality suitable for suprarenal vein sampling test on the basis of the amount of the labeled anti-cortisol antibodies determined in the above step.

摘要翻译： 一种用于评估血液样品质量的方法，包括以下步骤：将标记的抗皮质醇抗体与待进行血液样品混合，进行标记的抗皮质醇抗体与血液中含有的皮质醇的抗原 - 抗体反应 样品，将在上述步骤中获得的混合物在具有固定有皮质醇的底物的免疫色谱试纸上培养，引起与固定在底物上的皮质醇混合物中游离的标记抗皮质醇抗体的抗原 - 抗体反应 从而将抗体结合到皮质醇上，确定在上述步骤中与皮质醇结合的标记的抗皮质醇抗体的量，并且基于上述步骤评估血液样品是否具有适合于肾上静脉取样试验的质量 在上述步骤中测定的标记的抗皮质醇抗体的量。

公开(公告)号：US20110244476A1

公开(公告)日：2011-10-06

申请号：US12744064

申请日：2008-11-25

申请人： Koutarou Idegami ,  Takashi Yoneda ,  Yuzuru Takamura

发明人： Koutarou Idegami ,  Takashi Yoneda ,  Yuzuru Takamura

IPC分类号： G01N33/74 ,  G01N33/53

CPC分类号： G01N33/54366 ,  G01N33/558 ,  G01N33/9493

摘要： A method for evaluating the quality of a blood sample, comprising the steps of: mixing labeled anti-cortisol antibodies with a blood sample to be subjected, to conduct the antigen-antibody reaction of the labeled anti-cortisol antibody with cortisol contained in the blood sample, developing a mixture obtained in the above step on an immunochromatographic test strip having a substrate on which cortisol is immobilized to cause the antigen-antibody reaction of the labeled anti-cortisol antibodies which are free in the mixture with the cortisol immobilized on the substrate, thereby bonding the antibody to the cortisol, determining the amount of the labeled anti-cortisol antibodies bonded to the cortisol in the above step, and evaluating whether or not the blood sample has a quality suitable for suprarenal vein sampling test on the basis of the amount of the labeled anti-cortisol antibodies determined in the above step.

摘要翻译： 一种用于评估血液样品质量的方法，包括以下步骤：将标记的抗皮质醇抗体与待进行血液样品混合，进行标记的抗皮质醇抗体与血液中包含的皮质醇的抗原 - 抗体反应 样品，将在上述步骤中获得的混合物在具有固定有皮质醇的底物的免疫色谱试纸上培养，引起与固定在底物上的皮质醇混合物中游离的标记抗皮质醇抗体的抗原 - 抗体反应 从而将抗体结合到皮质醇上，确定在上述步骤中与皮质醇结合的标记的抗皮质醇抗体的量，并且基于上述步骤评估血液样品是否具有适合于肾上静脉取样试验的质量 在上述步骤中测定的标记的抗皮质醇抗体的量。