公开(公告)号：US07541515B2

公开(公告)日：2009-06-02

申请号：US10252732

申请日：2002-09-23

申请人： Elizabeth Hood ,  John Howard ,  Donna Delaney

发明人： Elizabeth Hood ,  John Howard ,  Donna Delaney

IPC分类号： C12N15/82

CPC分类号： C12N15/8216 ,  C12N15/8257

摘要： Increased expression of heterologous proteins in a plant is achieved by introducing the nucleotide sequence encoding the heterologous protein into a plant that has high oil seed content compared to low oil seed and/or is introduced into a plant having reduced alcohol soluble proteins in the endosperm. The nucleotide sequence may be introduced by direct transformation into the plant, or by direct transformation into another plant, and crossing with the high oil plant or plant have reduced levels of alcohol soluble protein in the endosperm. Further, the nucleotide sequences may be introduced into one or both of the high oil plant and the plant having reduced levels of alcohol soluble proteins, and the plants may be crossed to result in a progeny having even further increased expression levels of the heterologous protein.

摘要翻译： 通过将编码异源蛋白质的核苷酸序列引入与低油种子相比具有高油种子含量的植物和/或被引入具有在胚乳中具有降低的可溶性蛋白质的植物中来实现植物中异源蛋白质的表达增加。 核苷酸序列可以通过直接转化到植物中，或通过直接转化到另一种植物中，并与高油植物或植物杂交在胚乳中具有降低的可溶性蛋白质水平。 此外，可以将核苷酸序列引入高油植物和具有降低的可溶性蛋白质水平的植物中的一个或两个中，并且可以使植物杂交以产生具有甚至进一步增加异源蛋白质表达水平的后代。