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    Method of increasing expression of heterologous proteins in plants
    1.
    发明授权
    Method of increasing expression of heterologous proteins in plants 有权
    增加植物中异源蛋白表达的方法

    公开(公告)号：US07541515B2

    公开(公告)日：2009-06-02

    申请号：US10252732

    申请日：2002-09-23

    申请人： Elizabeth Hood ,  John Howard ,  Donna Delaney

    发明人： Elizabeth Hood ,  John Howard ,  Donna Delaney

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8216 ,  C12N15/8257

    摘要： Increased expression of heterologous proteins in a plant is achieved by introducing the nucleotide sequence encoding the heterologous protein into a plant that has high oil seed content compared to low oil seed and/or is introduced into a plant having reduced alcohol soluble proteins in the endosperm. The nucleotide sequence may be introduced by direct transformation into the plant, or by direct transformation into another plant, and crossing with the high oil plant or plant have reduced levels of alcohol soluble protein in the endosperm. Further, the nucleotide sequences may be introduced into one or both of the high oil plant and the plant having reduced levels of alcohol soluble proteins, and the plants may be crossed to result in a progeny having even further increased expression levels of the heterologous protein.

    摘要翻译： 通过将编码异源蛋白质的核苷酸序列引入与低油种子相比具有高油种子含量的植物和/或被引入具有在胚乳中具有降低的可溶性蛋白质的植物中来实现植物中异源蛋白质的表达增加。 核苷酸序列可以通过直接转化到植物中，或通过直接转化到另一种植物中，并与高油植物或植物杂交在胚乳中具有降低的可溶性蛋白质水平。 此外，可以将核苷酸序列引入高油植物和具有降低的可溶性蛋白质水平的植物中的一个或两个中，并且可以使植物杂交以产生具有甚至进一步增加异源蛋白质表达水平的后代。

    Monocotyledonous seed expressing exo-1,4B-glucanase
    2.
    发明授权
    Monocotyledonous seed expressing exo-1,4B-glucanase 有权
    表达外切-14B-葡聚糖酶的单子叶种子

    公开(公告)号：US08558058B2

    公开(公告)日：2013-10-15

    申请号：US11219180

    申请日：2005-09-02

    申请人： Elizabeth Hood ,  John Howard

    发明人： Elizabeth Hood ,  John Howard

    IPC分类号： C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8242 ,  C12N15/8246 ,  C12N15/8257

    摘要： Expression of recombinant polysaccharide degrading enzymes in plants is described. In one embodiment, expression of the enzyme is preferentially directed to the seed of the plant. Expression may also be preferentially targeted to specific locations within the plant cell. Expression of cellulases in corn is shown. The result is the capacity to produce polysaccharide degrading enzymes in plants at commercially acceptable levels in a reliable manner. Methods of using same in production of ethanol is also described, including use of the plant-produced enzymes in the ethanol production process.

    摘要翻译： 描述了重组多糖降解酶在植物中的表达。 在一个实施方案中，酶的表达优先针对植物的种子。 表达也可以优先靶向植物细胞内的特定位置。 显示玉米纤维素酶的表达。 结果是以可靠的方式以商业上可接受的水平在植物中产生多糖降解酶的能力。 还描述了在乙醇生产中使用其的方法，包括在乙醇生产过程中使用植物生产的酶。

    Commerical production of proteases in plants
    4.
    发明申请
    Commerical production of proteases in plants 审中-公开
    植物中商业化生产蛋白酶

    公开(公告)号：US20060015971A1

    公开(公告)日：2006-01-19

    申请号：US11226788

    申请日：2005-09-14

    申请人： John Howard ,  Elizabeth Hood

    发明人： John Howard ,  Elizabeth Hood

    IPC分类号： A01H1/00 ,  C12N15/82

    CPC分类号： C12N9/6427 ,  C12N15/8257

    摘要： Production of proteases in plants is set forth, whereby heterologous DNA encoding the protease is introduced into the plant and expression of the protein achieved. By such methods, expression is achieved in plants wherein the plant cell is not damaged, the protein can be recovered without contamination by other proteases, and can be expressed at levels such that commercial production of the enzyme is obtained. Expression levels can be at 0.1% of total soluble protein of the plant, or higher.

    摘要翻译： 阐述植物中蛋白酶的产生，由此将编码蛋白酶的异源DNA引入植物中并实现蛋白质的表达。 通过这样的方法，在植物细胞未受损害的植物中实现表达，可以在不受其它蛋白酶污染的情况下回收蛋白质，并且可以以获得酶的商业生产的水平来表达。 表达水平可以在植物总可溶性蛋白质的0.1％以上。

    RANDOM, SEQUENTIAL, OR SIMULTANEOUS MULTI-BEAM CIRCULAR ANTENNA ARRAY AND BEAM FORMING NETWORKS WITH UP TO 360° COVERAGE
    6.
    发明公开
    RANDOM, SEQUENTIAL, OR SIMULTANEOUS MULTI-BEAM CIRCULAR ANTENNA ARRAY AND BEAM FORMING NETWORKS WITH UP TO 360° COVERAGE 审中-公开

    公开(公告)号：US20240235603A9

    公开(公告)日：2024-07-11

    申请号：US18386626

    申请日：2023-11-03

    申请人： John Howard

    发明人： John Howard

    IPC分类号： H04B1/58 ,  H04B1/04 ,  H04B7/0426

    CPC分类号： H04B1/583 ,  H04B1/0458 ,  H04B7/043

    摘要： A beam forming network system includes a first beam forming network having first and second ports, in which each of the first ports is operatively coupled to an antenna element; and a second beam forming network including third and fourth ports, in which each of the third ports is operatively coupled to one of the second ports using at least one of a phase shifter, attenuator, power divider, and/or hybrid coupler. A method of beam forming includes coupling each of the first ports associated with a first beam forming network operatively to one antenna element, and coupling each of the third ports associated with a second beam forming network operatively to one of the second ports associated with the first beam forming network using at least one of a phase shifter, attenuator, power divider, and/or hybrid coupler.

    Random, sequential, or simultaneous multi-beam circular antenna array and beam forming networks with up to 360° coverage
    7.
    发明授权
    Random, sequential, or simultaneous multi-beam circular antenna array and beam forming networks with up to 360° coverage 有权

    公开(公告)号：US11855680B2

    公开(公告)日：2023-12-26

    申请号：US16891244

    申请日：2020-06-03

    申请人： John Howard

    发明人： John Howard

    IPC分类号： H01Q21/20 ,  H04B1/58 ,  H04B1/04 ,  H04B7/0426

    CPC分类号： H04B1/583 ,  H04B1/0458 ,  H04B7/043

    摘要： A beam forming network system includes a first beam forming network having first and second ports, in which each of the first ports is operatively coupled to an antenna element; and a second beam forming network including third and fourth ports, in which each of the third ports is operatively coupled to one of the second ports using at least one of a phase shifter, attenuator, power divider, and/or hybrid coupler. A method of beam forming includes coupling each of the first ports associated with a first beam forming network operatively to one antenna element, and coupling each of the third ports associated with a second beam forming network operatively to one of the second ports associated with the first beam forming network using at least one of a phase shifter, attenuator, power divider, and/or hybrid coupler.

    Random, sequential, or simultaneous multi-beam circular antenna array and beam forming networks with up to 360° coverage
    8.
    发明授权
    Random, sequential, or simultaneous multi-beam circular antenna array and beam forming networks with up to 360° coverage 有权

    公开(公告)号：US10734733B2

    公开(公告)日：2020-08-04

    申请号：US14227634

    申请日：2014-03-27

    申请人： John Howard

    发明人： John Howard ,  Chuck Wah Fung

    IPC分类号： H01Q21/00 ,  H01Q3/40 ,  H01Q3/24 ,  H01Q3/26 ,  H01Q21/20

    摘要： An antenna array system provides simultaneous 360° coverage and includes Butler matrix beam forming networks connected to an antenna array, which includes narrow and/or broadband elements, and multiple transmitters, receivers, or transceivers to allow for 360° transmission and/or reception. The antenna array system can provide multiple beams, such as without limitation 8 or 16 beams, which can vary in beam crossing and/or overlap to provide simultaneous 360° coverage. An antenna array system includes a plurality of antenna elements configured in an array, a first Butler matrix operatively coupled to the plurality of antenna elements, and a second Butler matrix operatively coupled to the first Butler matrix. A method of providing simultaneous 360° coverage includes configuring a plurality of antenna elements in an array, coupling a first Butler matrix operatively to the plurality of antenna elements, and coupling a second Butler matrix operatively to the first Butler matrix.

    Ultra-broadband antenna array with constant beamwidth throughout operating frequency band
    9.
    发明授权
    Ultra-broadband antenna array with constant beamwidth throughout operating frequency band 有权

    公开(公告)号：US09905936B2

    公开(公告)日：2018-02-27

    申请号：US15388509

    申请日：2016-12-22

    申请人： John Howard

    发明人： John Howard ,  Chuck Wah Fung

    IPC分类号： H01Q21/06 ,  H01Q21/20 ,  H01Q21/30 ,  H01Q21/00

    CPC分类号： H01Q21/06 ,  H01Q13/085 ,  H01Q21/0087 ,  H01Q21/064 ,  H01Q21/205 ,  H01Q21/30 ,  Y10T29/49016

    摘要： An antenna array includes a plurality of antenna elements coupled in a common area and extending radially outward from the common area. At least one of the plurality of antenna elements includes a first antenna portion and a second antenna portion arranged in a configuration such that a gap is formed between the first antenna portion and the second antenna portion. The gap includes first spacing associated with a first operating frequency and a first operating wavelength, and a second spacing associated with a second operating frequency and a second operating wavelength. A proportion of the first spacing to the first wavelength is substantially equal to a proportion of the second spacing to the second wavelength, thereby providing a constant beamwidth over an operating frequency band. A method of arranging a plurality of antenna elements in an antenna array is also disclosed.