公开(公告)号：US5597719A

公开(公告)日：1997-01-28

申请号：US276151

申请日：1994-07-14

申请人： Ellen Freed ,  Rosamaria Ruggieri

发明人： Ellen Freed ,  Rosamaria Ruggieri

IPC分类号： C07K14/47 ,  C12N9/12

CPC分类号： C12N9/1205 ,  C07K14/4705 ,  C07K2319/00

摘要： Intermolecular interactions between Raf-1 and human 14-3-3 proteins which regulate Raf activity are identified. Compositions and method for identifying novel drugs which modulate Raf activity in vivo are provided.

摘要翻译： 鉴定了Raf-1与调节Raf活性的人14-3-3蛋白之间的分子间相互作用。 提供了用于鉴定在体内调节Raf活性的新药物的组合物和方法。

公开(公告)号：US06511825B1

公开(公告)日：2003-01-28

申请号：US09399588

申请日：1999-09-20

申请人： Rosamaria Ruggieri ,  Marinella Callow ,  Paul W. Diaz

发明人： Rosamaria Ruggieri ,  Marinella Callow ,  Paul W. Diaz

IPC分类号： C12P2106

CPC分类号： C12N9/1205 ,  C07K14/47

摘要： The present invention relates to an isolated SRK polypeptide, biologically-active polypeptide fragments thereof, and nucleic acids which code for it. This polypeptide has various activities in regulating cell signaling and signal transduction pathways, including, e.g., a protein kinase activity; an autophosphorylating activity; a cell survival promoting activity; a HAX-1 binding activity; an apoptosis suppression activity; a MAPKK stimulatory activity; a transcription modulatory activity, and a SRK-specific immunogenic activity. The invention relates to all aspects of SRK, or homologs thereof, including assays for modulators, activators, ligands, etc. The invention also relates to a cytolic or soluble HAX-1 which produces apoptosis when expressed in cells.

摘要翻译： 本发明涉及分离的SRK多肽，其生物活性多肽片段和编码它的核酸。 该多肽在调节细胞信号传导和信号转导途径中具有各种活性，包括例如蛋白激酶活性; 自磷酸化活性; 细胞存活促进活性; HAX-1结合活性; 凋亡抑制活性; MAPKK刺激活性; 转录调节活性和SRK特异性免疫原性活性。 本发明涉及SRK或其同系物的所有方面，包括调节剂，激活剂，配体等的测定。本发明还涉及当在细胞中表达时产生细胞凋亡的细胞或可溶性HAX-1。