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公开(公告)号：US11427814B2
公开(公告)日：2022-08-30
申请号：US17213169
申请日：2021-03-25
申请人： Encodia, Inc.
摘要： Provided herein are modified cleavases for removing amino acids from peptides, polypeptides, and proteins. Also provided are methods of using the modified cleavases for treating polypeptides, and kits comprising the modified cleavase. In some embodiments, the methods and the kits also include other components for macromolecule sequencing and/or analysis.
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公开(公告)号：US11788080B2
公开(公告)日：2023-10-17
申请号：US17850516
申请日：2022-06-27
申请人： Encodia, Inc.
摘要： Provided herein are modified cleavases for removing amino acids from peptides, polypeptides, and proteins. Also provided are methods of using the modified cleavases for treating polypeptides, and kits comprising the modified cleavase. In some embodiments, the methods and the kits also include other components for macromolecule sequencing and/or analysis.
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3.发明申请公开(公告)号：US20220127754A1
公开(公告)日：2022-04-28
申请号：US17423094
申请日：2020-01-17
申请人： Encodia, Inc.
发明人： Stephen Verespy, III , Mark S. Chee
摘要： The present disclosure relates to methods of accelerating reactions involving macromolecules, e.g., peptides, polypeptides, and proteins for sequencing and/or analysis. In some embodiments, the methods include the application of radiation, e.g., electromagnetic radiation or microwave energy. In some embodiments, the methods and uses are for modifying a polypeptide or a plurality of polypeptides (e.g., peptides and proteins) for sequencing and/or analysis that employ barcoding and nucleic acid encoding of molecular recognition events, and/or detectable labels.
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公开(公告)号：US11639366B2
公开(公告)日：2023-05-02
申请号：US17535516
申请日：2021-11-24
申请人： Encodia, Inc.
发明人： Robert C. James , Stephen Verespy, III , Eric Cunyu Zhou , Eric Okerberg , Kelsey Rose Schramma , Fei Huang
IPC分类号： C07C49/603 , C07K1/13 , G01N33/58 , G01N33/68 , C07K1/107 , C07D235/02
摘要： The present invention relates to methods to use cyclohexan-1,2-dione (CHD) groups to attach labels, linkers, and other molecules to a target compound comprising a CHD-reactive group such as a guanidine, amidine, urea, thiourea and the like. Methods of the invention include milder conditions than those previously known for promoting reaction of CHD with CHD-reactive groups, which makes the methods suitable for use with base-sensitive compounds and complex biomolecules. Methods of the invention are especially useful for attaching linking and labeling groups to a peptide that comprises at least one arginine residue, and can also be used to link such peptides to other target molecules such as nucleic acids. The invention also provides CHD-containing conjugation reagents and compositions comprising CHD-containing intermediates, and precursors useful for making CHD-containing compounds that can be used in the methods of the invention.
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公开(公告)号：US20230021091A1
公开(公告)日：2023-01-19
申请号：US17850516
申请日：2022-06-27
申请人： Encodia, Inc.
摘要： Provided herein are modified cleavases for removing amino acids from peptides, polypeptides, and proteins. Also provided are methods of using the modified cleavases for treating polypeptides, and kits comprising the modified cleavase. In some embodiments, the methods and the kits also include other components for macromolecule sequencing and/or analysis.
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公开(公告)号：US20220144885A1
公开(公告)日：2022-05-12
申请号：US17535516
申请日：2021-11-24
申请人： Encodia, Inc.
发明人： Robert C. James , Stephen Verespy, III , Eric Cunyu Zhou , Eric Okerberg , Kelsey Rose Schramma , Fei HUANG
摘要： The present invention relates to methods to use cyclohexan-1,2-dione (CHD) groups to attach labels, linkers, and other molecules to a target compound comprising a CHD-reactive group such as a guanidine, amidine, urea, thiourea and the like. Methods of the invention include milder conditions than those previously known for promoting reaction of CHD with CHD-reactive groups, which makes the methods suitable for use with base-sensitive compounds and complex biomolecules. Methods of the invention are especially useful for attaching linking and labeling groups to a peptide that comprises at least one arginine residue, and can also be used to link such peptides to other target molecules such as nucleic acids. The invention also provides CHD-containing conjugation reagents and compositions comprising CHD-containing intermediates, and precursors useful for making CHD-containing compounds that can be used in the methods of the invention.
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