公开(公告)号：US20090220988A1

公开(公告)日：2009-09-03

申请号：US12065210

申请日：2006-08-29

申请人： Eric Trinquet ,  Hervè Ansanay ,  Gérard Mathis

发明人： Eric Trinquet ,  Hervè Ansanay ,  Gérard Mathis

IPC分类号： G01N33/53 ,  C12Q1/02

CPC分类号： G01N33/542 ,  G01N33/6872 ,  Y10S435/968

摘要： The present invention relates to a method of revealing a biological process using a FRET measurement, which comprises the following steps: incorporating, into a measurement medium containing a lipid membrane, a biological entity X coupled with a first member of a pair of FRET partners and a second biological entity Y coupled with the second member of the pair of FRET partners, the energy-donating member of the pair of FRET partners having a long lifetime and the members of said pair of FRET partners being located on either side of the lipid membrane; exciting the measurement medium at the excitation wavelength of the energy-donating member; and measuring the FRET signal or the variations in said signal emitted in said culture medium.

摘要翻译： 本发明涉及使用FRET测定揭示生物过程的方法，其包括以下步骤：将含有脂质膜的生物体X与一对FRET伴侣的第一成员结合， 与该对FRET伴侣的第二成员结合的第二生物实体Y，该对FRET伴侣中的能量供给成员具有长寿命，并且所述一对FRET伴侣的成员位于脂质膜的任一侧 ; 在能量供给构件的激发波长下激发测量介质; 以及测量FRET信号或在所述培养基中发射的所述信号的变化。