公开(公告)号：US20180265538A1

公开(公告)日：2018-09-20

申请号：US15862485

申请日：2018-01-04

申请人： Etablissement Français du Sang ,  Centre National de la Recherche Scientifique (CNRS)

发明人： François Morvan ,  Albert Meyer ,  Jean-Jacques Vasseur ,  Julie Mayen ,  Carole Chaix ,  Carole Farre ,  Chantal Fournier-Wirth ,  Jean-François Cantaloube ,  Myriam Lereau

IPC分类号： C07H21/04 ,  C08F257/02 ,  C07F9/24 ,  C07C323/52 ,  C12N15/11

CPC分类号： C07H21/04 ,  C07C323/52 ,  C07F9/2408 ,  C08F257/02 ,  C12N15/11

摘要： The present invention relates to a thiol compound suitable for forming a chain of oligomers that can be grafted to an oligonucleotide. The invention also relates to an oligonucleotide grafted by such a compound, thus having one or more thiol functions, suitable for being immobilized on a gold surface or on a grafted surface.

公开(公告)号：US11078229B2

公开(公告)日：2021-08-03

申请号：US15862485

申请日：2018-01-04

申请人： Etablissement Français du Sang ,  Centre National de la Recherche Scientifique (CNRS)

发明人： François Morvan ,  Albert Meyer ,  Jean-Jacques Vasseur ,  Julie Mayen ,  Carole Chaix ,  Carole Farre ,  Chantal Fournier-Wirth ,  Jean-François Cantaloube ,  Myriam Lereau

IPC分类号： C07H21/04 ,  C12N15/11 ,  C07F9/24 ,  C07C323/52 ,  C08F257/02

摘要： The present invention relates to a thiol compound suitable for forming a chain of oligomers that can be grafted to an oligonucleotide. The invention also relates to an oligonucleotide grafted by such a compound, thus having one or more thiol functions, suitable for being immobilized on a gold surface or on a grafted surface.

公开(公告)号：US09896473B2

公开(公告)日：2018-02-20

申请号：US14390747

申请日：2013-04-04

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) ,  UNIVERSITE DE MONTPELLIER 1 ,  ETABLISSEMENT FRANÇAIS DU SANG ,  UNIVERSITE CLAUSE BERNARD LYON 1

发明人： François Morvan ,  Albert Meyer ,  Jean-Jacques Vasseur ,  Julie Mayen ,  Carole Chaix ,  Carole Farre ,  Chantal Fournier-Wirth ,  Jean-François Cantaloube ,  Myriam Lereau

IPC分类号： C07H21/04 ,  C07F9/24 ,  C07C323/52 ,  C08F257/02 ,  C12N15/11

CPC分类号： C07H21/04 ,  C07C323/52 ,  C07F9/2408 ,  C08F257/02 ,  C12N15/11

摘要： The present invention relates to a thiol compound suitable for forming a chain of oligomers that can be grafted to an oligonucleotide. The invention also relates to an oligonucleotide grafted by such a compound, thus having one or more thiol functions, suitable for being immobilized on a gold surface or on a grafted surface.

公开(公告)号：US20150158964A1

公开(公告)日：2015-06-11

申请号：US14390747

申请日：2013-04-04

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) ,  UNIVERSITE DE MONTPELLIER 1 ,  ETABLISSMENT FRANÇAIS DU SANG ,  UNIVERSITE CLAUDE BERNARD LYON 1

发明人： François Morvan ,  Albert Meyer ,  Jean-Jacques Vasseur ,  Julie Mayen ,  Carole Chaix ,  Carole Farre ,  Chantal Fournier-Wirth ,  Jean-François Cantaloube ,  Myriam Lereau

IPC分类号： C08F257/02 ,  C12N15/11 ,  C07C323/52

CPC分类号： C07H21/04 ,  C07C323/52 ,  C07F9/2408 ,  C08F257/02 ,  C12N15/11

摘要： The present invention relates to a thiol compound suitable for forming a chain of oligomers that can be grafted to an oligonucleotide. The invention also relates to an oligonucleotide grafted by such a compound, thus having one or more thiol functions, suitable for being immobilized on a gold surface or on a grafted surface.

摘要翻译： 本发明涉及适于形成可以接枝到寡核苷酸的低聚物链的硫醇化合物。 本发明还涉及由这种化合物接枝的寡核苷酸，因此具有一个或多个硫醇官能团，适用于固定在金表面或接枝表面上。

公开(公告)号：US20150232957A1

公开(公告)日：2015-08-20

申请号：US14390748

申请日：2013-04-04

申请人： ETABLISSEMENT FRANÇAIS DU SANG ,  CENTRE NATIONALE DE LA RECHERCHE SCIENTIFIQUE (CNRS) ,  UNIVERSITE DE MONTPELLIER 1 ,  UNIVERSITE CLAUDE BERNARD LYON 1

发明人： Chantal Fournier-Wirth ,  Myriam Lereau ,  Jean-François Cantaloube ,  Jean-Jacques Vasseur ,  François Morvan ,  Albert Meyer ,  Julie Mayen ,  Carole Chaix ,  Carole Farre

IPC分类号： C12Q1/70

CPC分类号： C12Q1/707 ,  C07H21/00 ,  C07H21/04 ,  C12Q2600/158

摘要： The present invention relates to a modified oligonucleotide having two or more thiol functions, which can be immobilized on a gold surface or on a grafted surface, in particular a surface comprising at least one carbon-carbon double bond or carbon-carbon triple bond or haloacetamide functions, preferably maleimide or acrylamide functions. The invention also relates to a method for detecting a nucleic acid in a biological sample comprising a step of detecting hybridization between a modified oligonucleotide and a target nucleic acid amplified from the biological sample. The invention relates more particularly to a method for detecting, genotyping or sequencing a pathogenic organism, preferably a virus.

摘要翻译： 本发明涉及具有两个或多个硫醇官能团的修饰的寡核苷酸，其可以固定在金表面或接枝表面上，特别是包含至少一个碳 - 碳双键或碳 - 碳三键或卤代乙酰胺 功能，优选马来酰亚胺或丙烯酰胺的功能。 本发明还涉及用于检测生物样品中的核酸的方法，其包括检测经修饰的寡核苷酸与从生物样品扩增的靶核酸之间的杂交的步骤。 本发明更具体地涉及用于检测，基因分型或测序病原生物体，优选病毒的方法。