公开(公告)号：US20210189366A1

公开(公告)日：2021-06-24

申请号：US17054526

申请日：2019-05-10

申请人： FEED RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Huiying LUO ,  Bin YAO ,  Weina LIU ,  Yuan GU ,  Tao TU ,  Yuan WANG ,  Yaru WANG ,  Huoqing HUANG ,  Yingguo BAI ,  Xiaoyun SU ,  Kun MENG

IPC分类号： C12N9/24 ,  C07K1/14 ,  C12N15/63

摘要： The present invention relates to mannanase PMan5A mutant having improved heat resistance, gene and application thereof. Said mutant is obtained by substitution the 93th histidine with tyrosine, the 94th phenylalanine with tyrosine, the 356th leucine with histidine, and/or the 389th alanine with proline. The thermal tolerance of the single site mutation mutant H93Y, L356H and A389P are greatly improved over that of the wild mannanase PMan5A, and the thermal tolerance of the combination mutants shows the stack effect of the single site mutation, demonstrating the amino acids at the sites of 93, 94, 356, and 389 play the important role for the thermal stability of the mannanase of GH5 family