公开(公告)号：US20210189366A1

公开(公告)日：2021-06-24

申请号：US17054526

申请日：2019-05-10

申请人： FEED RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Huiying LUO ,  Bin YAO ,  Weina LIU ,  Yuan GU ,  Tao TU ,  Yuan WANG ,  Yaru WANG ,  Huoqing HUANG ,  Yingguo BAI ,  Xiaoyun SU ,  Kun MENG

IPC分类号： C12N9/24 ,  C07K1/14 ,  C12N15/63

摘要： The present invention relates to mannanase PMan5A mutant having improved heat resistance, gene and application thereof. Said mutant is obtained by substitution the 93th histidine with tyrosine, the 94th phenylalanine with tyrosine, the 356th leucine with histidine, and/or the 389th alanine with proline. The thermal tolerance of the single site mutation mutant H93Y, L356H and A389P are greatly improved over that of the wild mannanase PMan5A, and the thermal tolerance of the combination mutants shows the stack effect of the single site mutation, demonstrating the amino acids at the sites of 93, 94, 356, and 389 play the important role for the thermal stability of the mannanase of GH5 family

公开(公告)号：US20210388411A1

公开(公告)日：2021-12-16

申请号：US17263562

申请日：2019-07-13

申请人： FEED RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Bin YAO ,  Xiaoyun SU ,  Fei GAO ,  Huiying LUO ,  Huoqing HUANG ,  Yingguo BAI ,  Yuan WANG ,  Tao TU ,  Yaru WANG ,  Kun MENG

IPC分类号： C12Q1/40 ,  C12N15/80 ,  C07K14/38 ,  C07K14/435 ,  G01N15/14

摘要： The present invention relates to the field of genetic engineering, particularly to a recombinant expression vector for rapidly screening the high expression strains and a method for rapidly screening high expression strains. In the invention, an exogenous red fluorescent protein and Aspergillus fumigatus cell surface protein localization signal are fused and expressed, and the fusion gene (DsRed-AfMP1) is integrated into the genome of Trichoderma reesei, so as to construct a strain displaying red fluorescent protein on the surface of Trichoderma reesei. By sorting Trichoderma reesei strains with red fluorescent protein on the surface by flow cytometry, genes beneficial to the improvement of cellulase activity can be quickly isolated.

公开(公告)号：US20210340514A1

公开(公告)日：2021-11-04

申请号：US17283565

申请日：2019-09-30

申请人： (FEED RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES)

发明人： Huiying LUO ,  Bin YAO ,  Yujie GUO ,  Tao Tu ,  Yuan WANG ,  Huoqing HUANG ,  Yingguo BAI ,  Xiaoyun SU ,  Yaru WANG ,  Kun MENG

IPC分类号： C12N9/34

摘要： The present invention relates to the field of genetic engineering, particularly to a glucoamylase TIGal5, gene and application thereof. Said glucoamylase comprises the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:2 and has the excellent enzymic properties, which can be applied to feed, food, and medicine industries, can be industrially produce with the genetic engineering technics.

公开(公告)号：US20210139864A1

公开(公告)日：2021-05-13

申请号：US16618375

申请日：2017-05-31

申请人： FEED RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Bin YAO ,  Xiaoyun SU ,  Huang Qin ,  Huiying LUO ,  Huoqing HUANG ,  Yingguo BAI ,  Yuan WANG ,  Yaru WANG ,  Rui MA ,  Tao TU ,  Jianshuang MA

IPC分类号： C12N9/08 ,  C12N15/63

摘要： The present invention provides use of a manganese peroxidase in the detoxification of mycotoxins, and specifically, the present invention provides five manganese peroxidases (MnP-1, MnP-2, MnP-4, MnP-5, and MnP-6), genes thereof, and uses thereof. The present invention provides five manganese peroxidases (MnP-1, MnP-2, MnP-4, MnP-5, and MnP-6) derived from lignocellulose degradation bacteria, the amino acid sequences thereof being as set forth in SEQ ID NO: 1, SEQ ID NO: 4, SEQ ID NO: 7, SEQ ID NO: 10, and SEQ ID NO: 13.