公开(公告)号：US20170248500A1

公开(公告)日：2017-08-31

申请号：US15515794

申请日：2014-10-20

Applicant: FOSS ANALYTICAL A/S

Inventor： Claus HOLM ,  Malin DAHL ,  Christer Zoffman BISGAARD

IPC: G01N1/40 ,  G01N1/34 ,  G01N30/60 ,  B01D11/02

CPC classification number: G01N1/40 ,  A23L5/23 ,  B01D11/02 ,  B01D11/0203 ,  G01N1/34 ,  G01N1/4055 ,  G01N15/1012 ,  G01N15/1459 ,  G01N30/60 ,  G01N33/543 ,  G01N33/569 ,  G01N2001/2893 ,  G01N2001/4061 ,  G01N2015/1006 ,  G01N2015/1018 ,  G01N2030/009 ,  G01N2030/062

Abstract: Determination of Analytes in a Sample Matrix by Solvent Extraction A method for the assay of one or more analytes in a sample matrix comprising the steps of: performing analyte extraction on the sample matrix, said analyte extraction comprising combining the sample matrix with a solvent for an extraction period which is less than that required for reaching equilibrium; and separating the analyte containing solvent from the sample matrix; next measuring a level of analyte present in the separated solvent; and then applying in a computer a calibration by which is established a mathematical relationship between levels of analyte extracted from each of a plurality of reference samples by means of the process employed above in the extraction for the sample matrix and a reference value of the levels of analyte for each reference sample to thereby derive a measure of the level of analyte in the sample matrix. Specifically a method to determine the amount of mycotoxins in cereal grain, especially OTA (ochratoxin A) and DON (deoxynivalenol) by mixing with a solvent comprising water alcohol mixture, with 20-40% ethanol by volume.