摘要:
A flow cytometer apparatus and methods for detecting and clearing a clog therein are disclosed. An example method for detecting a clog may include (i) detecting, via a fault detection system of a flow cytometer, a first plurality of events associated with a first aliquot from a first sample well, (ii) determining a count of the first plurality of events associated with the first aliquot, (iii) determining whether the count of the first plurality of events is below a minimum count tolerance and (iv) (a) if the count of the first plurality of events is below the minimum count tolerance, then determining that the flow cytometer has a clog, (b) if the count of the first plurality of events is equal to or above the minimum count tolerance, then detecting a second plurality of events associated with a second aliquot from a second sample well.
摘要:
A method for calibrating a dark field microcopy setup is disclosed. The method includes preparing a plurality of particle samples, each with a known concentration and particle size, the plurality having more than one particle size and, optionally, more than one refractive index and more than one diluent. For each sample in the plurality, the sample is measured in the setup and the scattered light intensity and number of particles is measured. From this data, a relationship between the scattered light intensity, particle size and calibrated investigated volume can be determined. The calibrated investigated volume is used to obtain the proper particle size distribution in a given diluent.
摘要:
Hydrogel particles and their use in cytometic applications are described. The hydrogel particles described herein are selectively tunable to have at least one optical property substantially similar to the at least one optical property of a target cell. In this regard, the hydrogel particles provided herein in one aspect, are used as a calibration reagent for the detection of a target cell in a sample.
摘要:
A flow cytometer apparatus and methods for detecting and clearing a clog therein are disclosed. An example method for detecting a clog may include (i) detecting, via a fault detection system of a flow cytometer, a first plurality of events associated with a first aliquot from a first sample well, (ii) determining a count of the first plurality of events associated with the first aliquot, (iii) determining whether the count of the first plurality of events is below a minimum count tolerance and (iv) (a) if the count of the first plurality of events is below the minimum count tolerance, then determining that the flow cytometer has a clog, (b) if the count of the first plurality of events is equal to or above the minimum count tolerance, then detecting a second plurality of events associated with a second aliquot from a second sample well.
摘要:
A flow cytometer apparatus and methods for detecting and clearing a clog therein are disclosed. An example method for detecting a clog may include (i) detecting, via a fault detection system of a flow cytometer, a first plurality of events associated with a first aliquot from a first sample well, (ii) determining a count of the first plurality of events associated with the first aliquot, (iii) determining whether the count of the first plurality of events is below a minimum count tolerance and (iv) (a) if the count of the first plurality of events is below the minimum count tolerance, then determining that the flow cytometer has a clog, (b) if the count of the first plurality of events is equal to or above the minimum count tolerance, then detecting a second plurality of events associated with a second aliquot from a second sample well.
摘要:
The present disclosure relates to compositions comprising a hydrogel particle with optical properties substantially similar to the optical properties of a target cell, and methods for their use.
摘要:
A method is provided of forming an imaging reference device for a biological material imaging system. A first region is provided in a substrate formed from an optically transmissive material which is an analogue of biological tissue. This first region is stained using a first biological tissue stain. A second region is provided in second substrate which is optically transmissive and an analogue of biological tissue. The second region is stained with a second biological tissue stain. The first and second regions are overlapped as a pair, such that light incident upon the pair is modulated by the respective first and second biological tissue stains of the respective regions. Multiple regions of the first and second type may be provided, stained to different degrees.
摘要:
A method validating a particulate monitoring device includes generating a final gas stream having a particulate material concentration that corresponds to a target particulate material concentration, using the particulate monitoring device to measure the particulate material concentration in the final gas stream, and evaluating an accuracy of the particulate monitoring device based on a comparison of the particulate concentration in the final gas stream that was measured by the particulate monitoring device with the target particulate material concentration.
摘要:
Provided herein are improved cell sorter systems and methods. Such systems and methods provide a self-stabilizing sorter jet to automate calibration, and address the issue of drift in cell sorting systems. The systems and methods presented make it possible to determine and set the charge delay interval automatically with circuitry in the cell sorter. These circuits can set, monitor, and adjust the time delay continuously, allowing for a completely automatic, autonomous, turn-key, self-stabilizing sorter jet.
摘要:
A microfluidic multiple channel particle analysis system which allows particles from a plurality of particle sources to be independently simultaneously entrained in a corresponding plurality of fluid streams for analysis and sorting into particle subpopulations based upon one or more particle characteristics.