Methods and Apparatus for Real-Time Detection and Clearing of a Clog

    公开(公告)号:US20180364144A1

    公开(公告)日:2018-12-20

    申请号:US16049899

    申请日:2018-07-31

    申请人: Intellicyt

    IPC分类号: G01N15/14 G01N15/10

    摘要: A flow cytometer apparatus and methods for detecting and clearing a clog therein are disclosed. An example method for detecting a clog may include (i) detecting, via a fault detection system of a flow cytometer, a first plurality of events associated with a first aliquot from a first sample well, (ii) determining a count of the first plurality of events associated with the first aliquot, (iii) determining whether the count of the first plurality of events is below a minimum count tolerance and (iv) (a) if the count of the first plurality of events is below the minimum count tolerance, then determining that the flow cytometer has a clog, (b) if the count of the first plurality of events is equal to or above the minimum count tolerance, then detecting a second plurality of events associated with a second aliquot from a second sample well.

    Methods and Apparatus for Real-Time Detection and Clearing of a Clog

    公开(公告)号:US20170138836A1

    公开(公告)日:2017-05-18

    申请号:US15411366

    申请日:2017-01-20

    申请人: Intellicyt

    IPC分类号: G01N15/14

    摘要: A flow cytometer apparatus and methods for detecting and clearing a clog therein are disclosed. An example method for detecting a clog may include (i) detecting, via a fault detection system of a flow cytometer, a first plurality of events associated with a first aliquot from a first sample well, (ii) determining a count of the first plurality of events associated with the first aliquot, (iii) determining whether the count of the first plurality of events is below a minimum count tolerance and (iv) (a) if the count of the first plurality of events is below the minimum count tolerance, then determining that the flow cytometer has a clog, (b) if the count of the first plurality of events is equal to or above the minimum count tolerance, then detecting a second plurality of events associated with a second aliquot from a second sample well.

    Methods and apparatus for real-time detection and clearing of a clog
    5.
    发明授权
    Methods and apparatus for real-time detection and clearing of a clog 有权
    用于实时检测和清除堵塞的方法和装置

    公开(公告)号:US09551644B2

    公开(公告)日:2017-01-24

    申请号:US14328755

    申请日:2014-07-11

    申请人: Intellicyt

    IPC分类号: G03C5/16 G01N15/14 G01N15/10

    摘要: A flow cytometer apparatus and methods for detecting and clearing a clog therein are disclosed. An example method for detecting a clog may include (i) detecting, via a fault detection system of a flow cytometer, a first plurality of events associated with a first aliquot from a first sample well, (ii) determining a count of the first plurality of events associated with the first aliquot, (iii) determining whether the count of the first plurality of events is below a minimum count tolerance and (iv) (a) if the count of the first plurality of events is below the minimum count tolerance, then determining that the flow cytometer has a clog, (b) if the count of the first plurality of events is equal to or above the minimum count tolerance, then detecting a second plurality of events associated with a second aliquot from a second sample well.

    摘要翻译: 公开了一种用于检测和清除其中堵塞的流式细胞仪装置和方法。 用于检测堵塞的示例性方法可以包括(i)经由流式细胞仪的故障检测系统检测与第一样品池相关联的第一等分试样的第一多个事件,(ii)确定第一多个样品的计数 (iii)确定所述第一多个事件的计数是否低于最小计数容限,以及(iv)(a)如果所述第一多个事件的计数低于所述最小计数容限, 然后确定流式细胞计数器具有堵塞,(b)如果第一多个事件的计数等于或高于最小计数容限,则从第二样本阱检测与第二等分试样相关联的第二多个事件。

    Method for forming an image reference device
    7.
    发明授权
    Method for forming an image reference device 有权
    图像参考装置的形成方法

    公开(公告)号:US09454691B2

    公开(公告)日:2016-09-27

    申请号:US14642305

    申请日:2015-03-09

    申请人: FFEI Limited

    摘要: A method is provided of forming an imaging reference device for a biological material imaging system. A first region is provided in a substrate formed from an optically transmissive material which is an analogue of biological tissue. This first region is stained using a first biological tissue stain. A second region is provided in second substrate which is optically transmissive and an analogue of biological tissue. The second region is stained with a second biological tissue stain. The first and second regions are overlapped as a pair, such that light incident upon the pair is modulated by the respective first and second biological tissue stains of the respective regions. Multiple regions of the first and second type may be provided, stained to different degrees.

    摘要翻译: 提供了一种形成用于生物材料成像系统的成像参考装置的方法。 第一区域设置在由作为生物组织的类似物的透光材料形成的基板中。 使用第一生物组织染色对该第一区进行染色。 第二区域设置在光学透射的第二基底和生物组织的类似物中。 第二区域被第二生物组织染色染色。 第一和第二区域重叠成一对,使得入射在该对上的光被各个区域的相应的第一和第二生物组织污渍调制。 可以提供第一和第二类型的多个区域,以不同程度染色。

    Cell sorter system and method
    9.
    发明授权
    Cell sorter system and method 有权
    细胞分选系统和方法

    公开(公告)号:US09200334B2

    公开(公告)日:2015-12-01

    申请号:US14009032

    申请日:2012-03-13

    申请人: Ger van den Engh

    发明人: Ger van den Engh

    摘要: Provided herein are improved cell sorter systems and methods. Such systems and methods provide a self-stabilizing sorter jet to automate calibration, and address the issue of drift in cell sorting systems. The systems and methods presented make it possible to determine and set the charge delay interval automatically with circuitry in the cell sorter. These circuits can set, monitor, and adjust the time delay continuously, allowing for a completely automatic, autonomous, turn-key, self-stabilizing sorter jet.

    摘要翻译: 本文提供了改进的细胞分选机系统和方法。 这种系统和方法提供自稳定分选机射流以自动校准,并解决细胞分选系统中漂移的问题。 所提出的系统和方法使得可以自动确定和设置电池分选器中的电路的充电延迟时间。 这些电路可以连续设置,监视和调整时间延迟,从而允许一个完全自动的,自主的,交钥匙的自稳定分拣机。