公开(公告)号：US20210198307A1

公开(公告)日：2021-07-01

申请号：US16771781

申请日：2018-12-14

Applicant: FOUNDATION FOR BIOMEDICAL RESEARCH AND INNOVATION AT KOBE

Inventor： Yo-ichi NABESHIMA ,  Yoko NABESHIMA ,  Chiaki ABE

IPC: C07K1/22 ,  C12P21/02

Abstract: The purpose of the present invention is to provide a method for producing active GcMAF more simply and at a high yield. The present invention is a method for producing active GcMAF comprising a step for culturing host cells which are transfected with a VDBP expression vector in serum-free medium. This culture is preferably a suspension culture. In addition, this method for producing active GcMAF is also characterized by not requiring an enzyme treatment step for deglycosylation.