公开(公告)号：US20200172862A1

公开(公告)日：2020-06-04

申请号：US16782772

申请日：2020-02-05

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Christopher W. McMAHON ,  Lauren E. LITTLE ,  Wen Bo WANG ,  Nathaniel A. ELLIOTT

IPC: C12N5/0793 ,  A61K38/18 ,  C12N5/0797

Abstract: Methods are provided, in some aspects, for differentiating pluripotent cells into midbrain dopaminergic (DA) neurons using a mono-SMAD inhibition or inhibition of SMAD signaling with only one SMAD inhibitor. In some embodiments, mono-SMAD inhibition utilizes a single inhibitor of bone morphogenic protein (BMP) for differentiating pluripotent cells into midbrain DA neurons.