公开(公告)号：US20150219638A1

公开(公告)日：2015-08-06

申请号：US14420122

申请日：2013-08-06

申请人： FUJIREBIO INC. ,  EIDIA Co., Ltd.

发明人： Kentaro Yamaguchi ,  Katsumi Aoyagi ,  Azusa Terao

IPC分类号： G01N33/543

CPC分类号： G01N33/54306 ,  C07K14/745 ,  G01N33/57438 ,  G01N33/86 ,  G01N2333/745

摘要： Disclosed are a PIVKA-II measurement method that achieves better serum-plasma correlation than conventional methods, and a reagent and a kit therefor. The PIVKA-II measurement method according to the present invention comprises measuring PIVKA-II in a sample by an immunoassay using a mixture of an anti-F1 antibody that specifically binds to prothrombin fragment F1 or an antigen-binding fragment thereof and an anti-F2 antibody that specifically binds to prothrombin fragment F2 or an antigen-binding fragment thereof; and an anti-PIVKA-II antibody that specifically binds to PIVKA-II or an antigen-binding fragment thereof.

摘要翻译： 公开了PIVKA-II测定方法，其比常规方法获得更好的血浆 - 血浆相关性，以及试剂和试剂盒。 根据本发明的PIVKA-II测量方法包括使用特异性结合凝血酶原片段F1或其抗原结合片段的抗-F1抗体和抗F2的混合物通过免疫测定在样品中测量PIVKA-II 特异性结合凝血酶原片段F2或其抗原结合片段的抗体; 和与PIVKA-II或其抗原结合片段特异性结合的抗PIVKA-II抗体。

公开(公告)号：US10539559B2

公开(公告)日：2020-01-21

申请号：US14420122

申请日：2013-08-06

申请人： FUJIREBIO INC. ,  SEKISUI MEDICAL CO., Ltd.

发明人： Kentaro Yamaguchi ,  Katsumi Aoyagi ,  Azusa Terao

IPC分类号： G01N33/543 ,  G01N33/86 ,  C07K14/745 ,  G01N33/574

摘要： Disclosed are a PIVKA-II measurement method that achieves better serum-plasma correlation than conventional methods, and a reagent and a kit therefor. The PIVKA-II measurement method according to the present invention comprises measuring PIVKA-II in a sample by an immunoassay using a mixture of an anti-F1 antibody that specifically binds to prothrombin fragment F1 or an antigen-binding fragment thereof and an anti-F2 antibody that specifically binds to prothrombin fragment F2 or an antigen-binding fragment thereof; and an anti-PIVKA-II antibody that specifically binds to PIVKA-II or an antigen-binding fragment thereof.