公开(公告)号：US08993345B2

公开(公告)日：2015-03-31

申请号：US14063666

申请日：2013-10-25

Applicant: Furukawa Electric Co., Ltd.

Inventor： Hideki Aizawa ,  Michio Ohkubo ,  Masataka Nishida

IPC: G01N33/532 ,  C07K17/14 ,  G01N33/543 ,  G01N33/552 ,  G01N33/558

CPC classification number: C07K17/14 ,  G01N33/54346 ,  G01N33/54353 ,  G01N33/552 ,  G01N33/558 ,  Y10T436/143333

Abstract: A method of producing functional molecule-containing silica nanoparticles on which a biomolecule is bonded, containing the steps of: allowing silica nanoparticles containing a functional molecule and having a thiol group on a surface thereof to coexist with a linker molecule having a maleimido group and a carboxyl group in an aprotic solvent, thereby allowing formation of a thioether bond between the thiol group and the maleimido group, and obtaining functional molecule-containing silica nanoparticles on which the linker molecule is bonded; and allowing the functional molecule-containing silica nanoparticles on which the linker molecule is bonded to coexist with carbodiimide and a biomolecule having an amino group in an aqueous solvent, thereby allowing formation of an amide bond between the carboxyl group active esterified by the carbodiimide, and the amino group of the biomolecule.

Abstract translation: 一种制备其上结合有生物分子的含有功能分子的二氧化硅纳米颗粒的方法，包括以下步骤：使含有功能分子的二氧化硅纳米粒子与其表面上的硫醇基团共存于具有马来酰亚胺基和 羧基在非质子溶剂中，从而在硫醇基和马来酰亚胺基之间形成硫醚键，并获得其上键合有连接分子的含功能分子的二氧化硅纳米颗粒; 并且使含有连接体分子的功能性分子的二氧化硅纳米粒子与碳二亚胺和在水性溶剂中具有氨基的生物分子共存，由此可以在由碳二亚胺酯化的羧基之间形成酰胺键，和 生物分子的氨基。

公开(公告)号：US20190011442A1

公开(公告)日：2019-01-10

申请号：US16066639

申请日：2015-12-28

Applicant: Ortho-Clinical Diagnostics Kabushiki Kaisha ,  Furukawa Electric Co., Ltd.

Inventor： Masayuki Fukushima ,  Masataka Nishida ,  Michio Ohkubo ,  Kazutomi Miyoshi

IPC: G01N33/543

Abstract: The purpose of the present invention is to provide: a method for detecting a substance of interest and a method for quantifying a substance of interest, whereby it becomes possible to detect or quantify the substance of interest properly using a reagent that can be produced with high yield; a kit; and a method for preparing a regent. A method for detecting a substance of interest in a sample comprises a step of mixing a first conjugate 10, a second conjugate 20 and the sample together, then placing the resultant mixture under conditions such that a stimulus-responsive substance 11 can agglomerate, and then determining the presence or absence of the occurrence of dispersion of the stimulus-responsive substance 11 or the occurrence of an event associating with the aforementioned dispersion, wherein the first conjugate 10 is a conjugate of a first substance that contains the stimulus-responsive substance 11 with a first affinity substance 13 for a substance of interest 50, and the second conjugate 20 is a conjugate of a second substance 21 that has a hydrophilic moiety or an electrically charged moiety with a second affinity substance 23 for the substance of interest 50. The second substance 21 contains particles having a specific gravity of 1.4 or more, and the first affinity substance 13 and the second affinity substance 23 can bond to the substance of interest 50 simultaneously at different sites in the substance of interest 50 from each other.

公开(公告)号：US20180348228A1

公开(公告)日：2018-12-06

申请号：US15779006

申请日：2016-11-25

Applicant: Ortho-Clinical Diagnostics Kabushiki Kaisha ,  Furukawa Electric Co., Ltd.

Inventor： Satoru Sugita ,  Masayuki Fukushima ,  Masataka Nishida ,  Michio Ohkubo ,  Kazutomi Miyoshi

IPC: G01N33/58 ,  G01N33/543 ,  G01N33/536

CPC classification number: G01N33/50 ,  G01N33/543

Abstract: Provided is a method by which the quantity of objects to be detected can be quickly determined with high sensitivity. The method of determining the quantity of objects to be detected in a specimen comprises: a mixing step for preparing a mixture by mixing the specimen and carrier particles that carry stimulus-responsive substances and a first affinity substance for the objects to be detected; a measurement step for placing the mixture under the condition that the stimulus-responsive substances aggregate, and measuring the particle diameter of a suspended solid in the mixture; and a determination step for determining, on the basis of the particle diameter, the quantity of the objects to be detected.

公开(公告)号：US10545143B2

公开(公告)日：2020-01-28

申请号：US15016028

申请日：2016-02-04

Applicant: FURUKAWA ELECTRIC CO., LTD.

Inventor： Masataka Nishida ,  Nobumitsu Yamanaka ,  Kazutomi Miyoshi ,  Michio Ohkubo

IPC: G01N33/552 ,  G01N33/58

Abstract: Silica particles having a thiol group on a surface thereof, and satisfying the following conditions (a) to (c): (a) a particle diameter is 20 to 1,000 nm; (b) a density of the thiol group on the surface of the silica particles is 0.002 to 0.2 piece/nm2; and (c) a ratio (B/A) in terms of an amount B (piece/particle) of the thiol group existing on the surface of the silica particles to an amount A of sulfur elements in the silica particles (the number of sulfur elements derived from thiol per silica particle) is 0.10 to 0.60.

公开(公告)号：US10203326B2

公开(公告)日：2019-02-12

申请号：US15088742

申请日：2016-04-01

Applicant: FURUKAWA ELECTRIC CO., LTD.

Inventor： Masayuki Fukushima ,  Michio Ohkubo ,  Kazutomi Miyoshi ,  Masataka Nishida

IPC: G01N33/543 ,  G01N21/64 ,  G01N33/552 ,  G01N33/58

Abstract: A method of detecting a target substance, containing the steps of: incorporating labeling particles into a test liquid containing an analyte; heating the test liquid; irradiating the test liquid with excitation light, and detecting the target substance contained in the test liquid depending on a state of light emission of the labeling particles; wherein an aggregation state of the labeling particles is changed by the heating step; and wherein the labeling particles have a thermoresponsive polymer on a surface of a composite particle containing a magnetic material and a fluorescent material, and further have a biomolecule having properties of binding with the target substance.