公开(公告)号：US09365629B2

公开(公告)日：2016-06-14

申请号：US12733836

申请日：2008-09-23

申请人： Fabio Parmeggiani ,  Riccardo Pellarin ,  Anders Peter Larsen ,  Gautham Varadamsetty ,  Michael Tobias Stumpp ,  Andreas Plückthun

发明人： Fabio Parmeggiani ,  Riccardo Pellarin ,  Anders Peter Larsen ,  Gautham Varadamsetty ,  Michael Tobias Stumpp ,  Andreas Plückthun

IPC分类号： C40B40/10 ,  C07K14/47 ,  C12N15/10

CPC分类号： C07K14/4703 ,  C12N15/1044

摘要： The invention relates to collections of target-specific designed binding proteins based on armadillo repeat proteins, and to a method of generating them. Designed armadillo repeat proteins are based on consensus sequences of single armadillo repeat units. These repeat proteins can be used as scaffolds for peptide recognition. Such a scaffold provides a binding mode that is in principle the same for every small recognizable unit, e.g. an amino acid or dipeptide, allowing a precise and modular recognition of a peptide in extended conformation. The method allows to generate a series of modules recognizing these simple units, and to combine such building blocks to create a binding site for any desired peptide target without performing additional selections.

摘要翻译： 本发明涉及基于犰狳重复蛋白的靶特异性设计的结合蛋白的集合，以及生成它们的方法。 设计的犰狳重复蛋白基于单犰狳重复单位的共有序列。 这些重复蛋白质可用作肽识别的支架。 这样的支架提供了对于每个小的可识别单元原则上相同的装订模式，例如， 氨基酸或二肽，允许在扩展构象中精确和模块识别肽。 该方法允许产生识别这些简单单元的一系列模块，并且组合这些构建块以产生任何所需肽靶的结合位点，而不进行附加选择。

公开(公告)号：US20110224100A1

公开(公告)日：2011-09-15

申请号：US12733836

申请日：2008-09-21

申请人： Fabio Parmeggiani ,  Riccardo Pellarin ,  Anders Peter Larsen ,  Gautham Varadamsetty ,  Michael Tobias Stumpp ,  Andreas Plückthun

发明人： Fabio Parmeggiani ,  Riccardo Pellarin ,  Anders Peter Larsen ,  Gautham Varadamsetty ,  Michael Tobias Stumpp ,  Andreas Plückthun

IPC分类号： C40B40/08 ,  C40B40/10 ,  C07K14/435 ,  C07K1/14

CPC分类号： C07K14/4703 ,  C12N15/1044

摘要： The invention relates to collections of target-specific designed binding proteins based on armadillo repeat proteins, and to a method of generating them. Designed armadillo repeat proteins are based on consensus sequences of single armadillo repeat units. These repeat proteins can be used as scaffolds for peptide recognition. Such a scaffold provides a binding mode that is in principle the same for every small recognizable unit, e.g. an amino acid or dipeptide, allowing a precise and modular recognition of a peptide in extended conformation. The method allows to generate a series of modules recognizing these simple units, and to combine such building blocks to create a binding site for any desired peptide target without performing additional selections.

摘要翻译： 本发明涉及基于犰狳重复蛋白的靶特异性设计的结合蛋白的集合，以及生成它们的方法。 设计的犰狳重复蛋白基于单犰狳重复单位的共有序列。 这些重复蛋白质可用作肽识别的支架。 这样的支架提供了对于每个小的可识别单元原则上相同的装订模式，例如， 氨基酸或二肽，允许在扩展构象中精确和模块识别肽。 该方法允许产生识别这些简单单元的一系列模块，并且组合这些构建块以产生任何所需肽靶的结合位点，而不进行附加选择。