公开(公告)号：US20100204468A1

公开(公告)日：2010-08-12

申请号：US12764537

申请日：2010-04-21

申请人： Faustinus YEBOAH ,  Mahmood PIRAEE

发明人： Faustinus YEBOAH ,  Mahmood PIRAEE

IPC分类号： C07D243/10 ,  C12P17/10 ,  C12N1/00 ,  C12N1/20

CPC分类号： C12P17/10 ,  B01D15/00 ,  B01D61/145 ,  C07D243/38 ,  C12N1/20 ,  C12P17/12 ,  C12R1/29

摘要： The present invention provides a scalable process for producing a concentrate containing a mass of a farnesylated dibenzodiazepinone by fermenting in an aqueous culture medium a strain of a microorganism that is capable of producing the farnesylated dibenzodiazepinone, upon completion of fermentation harvesting the fermentation broth and extracting the fermentation broth to provide an extract, and thereafter treating the extract to form the concentrate. The concentrate so produced may be utilized in downstream processes for producing pharmaceutical compounds. A strain of a Micromonospora species capable of producing a farnesylated dibenzodiazepinone at a high yield rate is provided, together with culture media for culturing microorganisms, and fermentation conditions for production of the farnesylated dibenzodiazepinone of the concentrate.

摘要翻译： 本发明提供了一种可扩展的方法，用于生产含有大量法呢基化二苯并二氮杂酮的浓缩物，其通过在水性培养基中发酵能够产生法呢基化二苯并二氮杂酮的微生物菌株，在完成发酵收获发酵液并提取 发酵液提供提取物，然后处理提取物以形成浓缩物。 如此生产的浓缩物可用于生产药物化合物的下游方法。 提供能够以高产率生产法呢基化二苯并二氮杂酮的小单孢菌属菌株，以及用于培养微生物的培养基和用于生产浓缩物的法呢基化二苯并二氮杂酮的发酵条件。