公开(公告)号：US20090176275A1

公开(公告)日：2009-07-09

申请号：US12331786

申请日：2008-12-10

申请人： Felix MALENFANT ,  Alaka MULLICK

发明人： Felix MALENFANT ,  Alaka MULLICK

IPC分类号： C12P21/02 ,  C07K14/32 ,  C07H21/00 ,  C12N15/74 ,  C12N5/10

CPC分类号： C07K14/21

摘要： Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from Pseudomonas putida. Disclosed herein are novel expression systems and components thereof, which involve the development of a CymR variant with reverse DNA binding activity, such that they exhibit increased affinity for DNA in a presence rather than an absence of an effector molecule such as cumene or an equivalent thereof. Also disclosed are the CymR variant, fusion proteins incorporating such a variant, and its use in the control and expression of polynucleotides. The CymR variant comprises a 142Glu to 142Gly single point mutation of wild type CymR.

摘要翻译： 最近，诱导型表达系统的发展涉及从恶臭假单胞菌（Pseudomonas putida）中开发p-cym操纵子。 本文公开了新的表达系统及其组分，其涉及开发具有反向DNA结合活性的CymR变体，使得它们在存在而不是不存在效应分子如枯烯或其等同物时表现出对DNA的增加的亲和力 。 还公开了CymR变体，掺入这种变体的融合蛋白及其在多核苷酸的控制和表达中的用途。 CymR变体包含野生型CymR的142Glu至142Gly单点突变。

公开(公告)号：US20110171686A1

公开(公告)日：2011-07-14

申请号：US13070975

申请日：2011-03-24

申请人： Félix MALENFANT ,  Alaka MULLICK

发明人： Félix MALENFANT ,  Alaka MULLICK

IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10

CPC分类号： C07K14/21

摘要： Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from Pseudomonas putida. Disclosed herein are novel expression systems and components thereof, which involve the development of a CymR variant with reverse DNA binding activity, such that they exhibit increased affinity for DNA in a presence rather than an absence of an effector molecule such as cumene or an equivalent thereof. Also disclosed are the CymR variant, fusion proteins incorporating such a variant, and its use in the control and expression of polynucleotides. The CymR variant comprises a 142Glu to 142Gly single point mutation of wild type CymR.

摘要翻译： 最近，诱导型表达系统的发展涉及从恶臭假单胞菌（Pseudomonas putida）中开发p-cym操纵子。 本文公开了新的表达系统及其组分，其涉及开发具有反向DNA结合活性的CymR变体，使得它们在存在而不是不存在效应分子如枯烯或其等同物时表现出对DNA的增加的亲和力 。 还公开了CymR变体，掺入这种变体的融合蛋白及其在多核苷酸的控制和表达中的用途。 CymR变体包含野生型CymR的142Glu至142Gly单点突变。