公开(公告)号：US20160168636A1

公开(公告)日：2016-06-16

申请号：US14882866

申请日：2015-10-14

Applicant: Fluidigm Corporation

Inventor： Suhaib Siddiqi ,  J. William Efcavitch ,  Judith Mitchell ,  Subramanian Marappan ,  Jayson Bowers ,  Mirna Jaroza

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  A61K49/0041 ,  A61K49/0052 ,  C07H19/06 ,  C07H19/16 ,  C12Q1/6869 ,  C12Q2563/107

Abstract: The invention generally relates to nucleotide analogs and methods of their use in sequencing-by-synthesis reactions. In certain embodiments, the invention provides a nucleotide analog including a detectable label attached to a nitrogenous base portion of a nucleotide analog by a cleavable linker, in which contact of the analog with at least one activating agent results in cleavage of the label and elimination of the linker, thereby producing a natural nucleotide, a 9-deaza-G, 9-deaza-A, or ψ-uridine.

Abstract translation: 本发明一般涉及核苷酸类似物及其在合成反应测序中的用途。 在某些实施方案中，本发明提供核苷酸类似物，其包括通过可切割接头连接到核苷酸类似物的含氮碱基部分的可检测标记，其中所述类似物与至少一种活化剂的接触导致标记的切割和消除 接头，从而产生天然核苷酸，9-脱氮-G，9-脱氮-A或ψ-尿苷。