公开(公告)号：US20100253762A1

公开(公告)日：2010-10-07

申请号：US12759839

申请日：2010-04-14

申请人： Fook Chiong CHEONG ,  David G. Grier ,  Sang-Hyuk Lee

发明人： Fook Chiong CHEONG ,  David G. Grier ,  Sang-Hyuk Lee

IPC分类号： H04N7/00 ,  G03H1/08 ,  G02B21/32 ,  H04N5/89

CPC分类号： G01B9/021 ,  G02B21/0088 ,  G02B21/32 ,  G03H1/0005 ,  G03H1/0443 ,  G03H1/0866 ,  G03H1/2294 ,  G03H2001/0033 ,  G03H2001/0077 ,  G03H2001/0447

摘要： A method and system for performing three-dimensional holographic microscopy of an optically trapped one dimensional structure. The method and system use an inverted optical microscope, a laser source which generates a trapping laser beam wherein the laser beam is focused by an objective lens into a plurality of optical traps. The method and system also use a collimated laser at an imaging wavelength to illuminate the structure created by the optical traps. Imaging light scattered by the optically tapped structure forms normalized intensity holograms that are imaged by a video camera and analyzed by optical formalisms to determine light field to reconstruct 3-D images for analysis and evaluation.

摘要翻译： 用于执行光学捕获的一维结构的三维全息显微镜的方法和系统。 该方法和系统使用倒置的光学显微镜，产生捕获激光束的激光源，其中激光束被物镜聚焦成多个光阱。 该方法和系统还使用成像波长处的准直激光来照亮由光阱产生的结构。 通过光学抽头结构散射的成像光形成归一化的强度全息图，其由摄像机成像并通过光学形式进行分析，以确定光场以重建用于分析和评估的3-D图像。