公开(公告)号：US20230148412A1

公开(公告)日：2023-05-11

申请号：US17812415

申请日：2022-07-13

Applicant: Foundation Medicine, Inc.

Inventor： Doron LIPSON ,  Geoffrey Alan OTTO ,  Alexander Nevin PARKER ,  Philip James STEPHENS ,  Sean R. DOWNING ,  Mirna JAROSZ ,  Mikhail G. SHAPIRO ,  Roman YELENSKY

IPC: C12Q1/6827 ,  C12Q1/6874 ,  G16B20/10 ,  G16B30/10 ,  G16B20/20 ,  G16B20/00 ,  G16B30/00

CPC classification number: C12Q1/6827 ,  C12Q1/6874 ,  G16B20/10 ,  G16B30/10 ,  G16B20/20 ,  G16B20/00 ,  G16B30/00 ,  C12Q1/6886

Abstract: A method of analyzing a tumor sample comprising:



(a) acquiring a library comprising a plurality of tumor members from a tumor sample;
(b) contacting the library with a bait set to provide selected members;
(c) acquiring a read for a subgenomic interval from a tumor member from said library;
(d) aligning said read; and
(e) assigning a nucleotide value (e.g., calling a mutation) from said read for the preselected nucleotide position, thereby analyzing said tumor sample.