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公开(公告)号：US20100068725A1
公开(公告)日：2010-03-18
申请号：US12517754
申请日：2008-01-30
CPC分类号： G01N33/82
摘要： A method for quantitating vitamin D metabolites directly in blood plasma or serum, without the need for prior purification of the vitamin D metabolites, comprising a digestion of the serum proteins with a serine protease such as proteinase K and sequence of steps for inhibiting the proteinase K activity in the competitive binding analysis. The advantages of this method are its high accuracy over the whole range of physiologically relevant values and that it can be easily adapted for a fully automated analysis of serum and plasma samples.
摘要翻译： 一种在血浆或血清中直接定量维生素D代谢物的方法，不需要先前纯化维生素D代谢物，包括用丝氨酸蛋白酶如蛋白酶K消化血清蛋白和抑制蛋白酶K的步骤顺序 竞争性结合分析中的活动。 该方法的优点是在生理相关值的整个范围内具有高精度，并且可以容易地适应于血清和血浆样品的全自动分析。
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公开(公告)号：US07964363B2
公开(公告)日：2011-06-21
申请号：US12517754
申请日：2008-01-30
IPC分类号： G01N33/53
CPC分类号： G01N33/82
摘要： A method for quantitating vitamin D metabolites directly in blood plasma or serum, without the need for prior purification of the vitamin D metabolites, comprising a digestion of the serum proteins with a serine protease such as proteinase K and sequence of steps for inhibiting the proteinase K activity in the competitive binding analysis. The advantages of this method are its high accuracy over the whole range of physiologically relevant values and that it can be easily adapted for a fully automated analysis of serum and plasma samples.
摘要翻译： 一种在血浆或血清中直接定量维生素D代谢物的方法，不需要先前纯化维生素D代谢物，包括用丝氨酸蛋白酶如蛋白酶K消化血清蛋白和抑制蛋白酶K的步骤顺序 竞争性结合分析中的活动。 该方法的优点是在生理相关值的整个范围内具有高精度，并且可以容易地适应于血清和血浆样品的全自动分析。
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