COMPOSITE PRIMER SET AND KIT FOR IDENTIFYING POLYMORPHIC MARKERS OF CATTLE AND APPLICATIONS THEREOF

    公开(公告)号:US20240360520A1

    公开(公告)日:2024-10-31

    申请号:US18354652

    申请日:2023-07-19

    CPC classification number: C12Q1/6888 C12Q2600/156 C12Q2600/16

    Abstract: A primer composition, a kit and a method for detecting polymorphic STR markers based on capillary electrophoresis and applications thereof are provided, which are used to simultaneously amplify 13 STR loci on cattle genome. The primer composition includes one or more pairs of primers with sequences as shown in SEQ ID NO: 1˜26. Developmental validation indicated that the kit is of high sensitivity, fine specificity, strong stability and high accuracy. This provides an effective tool for species identification, individual identification and parental analysis of cattle samples in civil disputes and illegal crimes.

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