公开(公告)号：US20250110120A1

公开(公告)日：2025-04-03

申请号：US18833026

申请日：2022-01-31

Applicant: GENESPECTOR S.R.O. ,  WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Stanislav KMOCH ,  Martina ZIVNA ,  Jan ZIVNY ,  Helena TRESLOVA ,  Anthony BLEYER ,  Kendrah KIDD

IPC: G01N33/543 ,  G01N33/68

Abstract: Method for determination of mutated form of mucin-1 protein in a biological sample comprising steps of adding biological sample to immobilized antibody against mutated form of VNTR MUC1fs, adding antibody against wild-type VNTR MUC1fs to portion of this mixture and performing quantitative analysis, wherein antibody against mutated form of VNTR MUC1fs is added to remainder of mixture of biological sample with immobilized antibody against mutated form of VNTR MUC1fs quantitative analysis is performed. Determination of concentration of MUC1fs in sample and determination of length of mutated or wild-type VNTR MUC1fs in sample is performed using appropriate calibration curves obtained by analyzing standard containing at least one epitope of mutated and at least one epitope of wild-type VNTR MUC1fs.