公开(公告)号：US20200216887A1

公开(公告)日：2020-07-09

申请号：US16071406

申请日：2017-01-20

Applicant: Genia Technologies, Inc. ,  ROCHE DIAGNOSTICS GMBH

Inventor： Timothy Kellogg Craig ,  Christos Tzitzilonis ,  Alexander H. Yang ,  Liv E. Jensen ,  Marshall Porter ,  Charlotte Yang ,  Corissa Harris ,  Matt Dipetro

IPC: C12Q1/6869 ,  C12N9/12

Abstract: A method is provided for preparing nanopore sequencing complexes in membranes for sequencing of polymers, e.g., polynucleotides and polypeptides. The nanopore sequencing complex is formed by the sequential linking of an enzyme to a nanopore that is inserted in a membrane, and of a polymer to the enzyme. Alternatively, the nanopore sequencing complex is formed by linking a preformed enzyme-polymer complex to a nanopore that is inserted in a membrane. The enzyme polymer complex is interchangeable.

公开(公告)号：US20190079067A1

公开(公告)日：2019-03-14

申请号：US16146053

申请日：2018-09-28

Applicant: GENIA TECHNOLOGIES, INC.

Inventor： Timothy Craig ,  Corissa Harris ,  Matt Dipetro ,  Mark Ambroso ,  Yaozhong Zou ,  Marshall Porter ,  Issa Delkaninia ,  Alexxa Noble ,  James Fairman ,  Wiseley Wu ,  Kapil Bajaj ,  Giovanni Bellesia ,  Seong-Ho Shin

IPC: G01N33/487 ,  C07K14/31

Abstract: Described herein are nanopore protein conjugates that can be used in DNA sequencing reactions. The nanopore protein conjugates includes a nanopore protein monomer that is joined to a DNA binding domain. The nanopore protein monomer is available to oligomerize with other nanopore protein monomers, while the DNA binding domain is available to bind to a template DNA strand. In certain examples, the nanopore protein monomer is an alpha-hemolysin monomer or variant thereof and the DNA binding domain is an Sso7d protein or variant thereof, such as an Sso7d-like protein. Also provided are nanopore protein assemblies incorporating the nanopore protein conjugates, along with methods of using the nanopore protein assemblies in sequencing reactions.