公开(公告)号：US20190079067A1

公开(公告)日：2019-03-14

申请号：US16146053

申请日：2018-09-28

Applicant: GENIA TECHNOLOGIES, INC.

Inventor： Timothy Craig ,  Corissa Harris ,  Matt Dipetro ,  Mark Ambroso ,  Yaozhong Zou ,  Marshall Porter ,  Issa Delkaninia ,  Alexxa Noble ,  James Fairman ,  Wiseley Wu ,  Kapil Bajaj ,  Giovanni Bellesia ,  Seong-Ho Shin

IPC: G01N33/487 ,  C07K14/31

Abstract: Described herein are nanopore protein conjugates that can be used in DNA sequencing reactions. The nanopore protein conjugates includes a nanopore protein monomer that is joined to a DNA binding domain. The nanopore protein monomer is available to oligomerize with other nanopore protein monomers, while the DNA binding domain is available to bind to a template DNA strand. In certain examples, the nanopore protein monomer is an alpha-hemolysin monomer or variant thereof and the DNA binding domain is an Sso7d protein or variant thereof, such as an Sso7d-like protein. Also provided are nanopore protein assemblies incorporating the nanopore protein conjugates, along with methods of using the nanopore protein assemblies in sequencing reactions.