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公开(公告)号:US06531583B1
公开(公告)日:2003-03-11
申请号:US09601198
申请日:2000-12-08
申请人: Gail H. Cassell , Ellson Y. Chen , Jennifer S. Glass , John I. Glass , Cheryl R. Heiner , Elliot Lefkowitz
发明人: Gail H. Cassell , Ellson Y. Chen , Jennifer S. Glass , John I. Glass , Cheryl R. Heiner , Elliot Lefkowitz
IPC分类号: C07H1900
CPC分类号: C12Q1/689
摘要: A method of detecting a nucleic acid sequence in Ureaplasma urealyticum serovar 3 includes the steps of isolating a specimen containing nucleic acid; and analyzing the specimen with an assay such as in situ hybridization, Southern blotting, single strand conformational polymorphism, restriction endonuclease fingerprinting (REF), PCR amplification, 5′ nuclease assay (TaqMan PCR), and DNA-chip analysis using the nucleic acid sequences SEQ ID Nos:1-181.
摘要翻译: 解脲脲原体血清型3中的核酸序列的检测方法包括:分离含有核酸的标本的步骤; 并使用原位杂交,Southern印迹,单链构象多态性,限制性内切核酸酶指纹图谱(REF),PCR扩增,5'核酸酶测定(TaqMan PCR)和使用核酸序列的DNA芯片分析 SEQ ID NO:1-181。