公开(公告)号：US20190040435A1

公开(公告)日：2019-02-07

申请号：US15994950

申请日：2018-05-31

Applicant: Genentech, Inc.

Inventor： Meixia ZHOU ,  Bradley Richard SNEDECOR ,  Chi Kin Domingos NG ,  Amy SHEN

IPC: C12P21/00 ,  C12N15/113 ,  C12N1/38 ,  C12N15/85 ,  C12N15/11

CPC classification number: C12P21/00 ,  C12N1/38 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/85 ,  C12N2310/14 ,  C12N2320/00 ,  C12Y101/01027 ,  C12Y102/04001

Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.

公开(公告)号：US20170191102A1

公开(公告)日：2017-07-06

申请号：US15268424

申请日：2016-09-16

Applicant: Genentech, Inc.

Inventor： Meixia ZHOU ,  Bradley Richard SNEDECOR ,  Chi Kin Domingos NG ,  Amy SHEN

IPC: C12P21/00 ,  C12N15/113

CPC classification number: C12P21/00 ,  C12N1/38 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/85 ,  C12N2310/14 ,  C12N2320/00 ,  C12Y101/01027 ,  C12Y102/04001

Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.

公开(公告)号：US20200377919A1

公开(公告)日：2020-12-03

申请号：US16888344

申请日：2020-05-29

Applicant: Genentech, Inc.

Inventor： Meixia ZHOU ,  Bradley Richard SNEDECOR ,  Chi Kin Domingos NG ,  Amy SHEN

IPC: C12P21/00 ,  C12N15/113 ,  C12N1/38 ,  C12N15/11 ,  C12N15/85

Abstract: The present invention provides ethods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.