公开(公告)号：US20200377919A1

公开(公告)日：2020-12-03

申请号：US16888344

申请日：2020-05-29

Applicant: Genentech, Inc.

Inventor： Meixia ZHOU ,  Bradley Richard SNEDECOR ,  Chi Kin Domingos NG ,  Amy SHEN

IPC: C12P21/00 ,  C12N15/113 ,  C12N1/38 ,  C12N15/11 ,  C12N15/85

Abstract: The present invention provides ethods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.

公开(公告)号：US20210214462A1

公开(公告)日：2021-07-15

申请号：US17148456

申请日：2021-01-13

Applicant: Genentech, Inc.

Inventor： Shahram MISAGHI ,  John B. LOWE ,  Bradley Richard SNEDECOR

IPC: C07K16/44 ,  C07K14/435 ,  G01N33/68

Abstract: The present application relates to methods of producing a protein in fucosydated and afucosylated forms at a predetermined ratio.

公开(公告)号：US20180251572A1

公开(公告)日：2018-09-06

申请号：US15968214

申请日：2018-05-01

Applicant: Genentech, Inc.

Inventor： Shahram MISAGHI ,  John B. LOWE ,  Bradley Richard SNEDECOR

IPC: C07K16/44 ,  G01N33/68

CPC classification number: C07K16/44 ,  C07K14/435 ,  C07K2317/41 ,  C07K2317/52 ,  C07K2317/732 ,  C07K2319/00 ,  G01N33/6854

Abstract: The present application relates to methods of producing a protein in fucosylated and afucosylated forms at a predetermined ratio.

公开(公告)号：US20230159625A1

公开(公告)日：2023-05-25

申请号：US18156215

申请日：2023-01-18

Applicant: Genentech, Inc.

Inventor： Benjamin HALEY ,  Zhilan HU ,  John C. JOLY ,  Amy Y. SHEN ,  Bradley Richard SNEDECOR

IPC: C07K16/00 ,  C12N15/113 ,  C12N15/85 ,  C12P21/02

CPC classification number: C07K16/00 ,  C12N15/1137 ,  C12N15/85 ,  C12P21/02 ,  C07K2317/14 ,  C07K2317/52 ,  C12N2310/14 ,  C12N2510/02 ,  C12N2510/00 ,  C12N2511/00 ,  C12N2310/20 ,  C07K2319/30

Abstract: The present invention provides compositions comprising a Fc-containing protein wherein substantially all the Fc domains have a C-terminal lysine. Further provided are host cell for producing said compositions, methods of making said host cells and compositions, and method of use thereof.

公开(公告)号：US20170191102A1

公开(公告)日：2017-07-06

申请号：US15268424

申请日：2016-09-16

Applicant: Genentech, Inc.

Inventor： Meixia ZHOU ,  Bradley Richard SNEDECOR ,  Chi Kin Domingos NG ,  Amy SHEN

IPC: C12P21/00 ,  C12N15/113

CPC classification number: C12P21/00 ,  C12N1/38 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/85 ,  C12N2310/14 ,  C12N2320/00 ,  C12Y101/01027 ,  C12Y102/04001

Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.

公开(公告)号：US20240158536A1

公开(公告)日：2024-05-16

申请号：US18474014

申请日：2023-09-25

Applicant: Genentech, Inc.

Inventor： Shahram MISAGHI ,  John B. LOWE ,  Bradley Richard SNEDECOR

IPC: C07K16/44 ,  C07K14/435 ,  G01N33/68

CPC classification number: C07K16/44 ,  C07K14/435 ,  G01N33/6854 ,  C07K2317/41 ,  C07K2317/52 ,  C07K2317/732 ,  C07K2319/00

Abstract: The present application relates to methods of producing a protein in fucosylated and afucosylated forms at a predetermined ratio.

公开(公告)号：US20190040435A1

公开(公告)日：2019-02-07

申请号：US15994950

申请日：2018-05-31

Applicant: Genentech, Inc.

Inventor： Meixia ZHOU ,  Bradley Richard SNEDECOR ,  Chi Kin Domingos NG ,  Amy SHEN

IPC: C12P21/00 ,  C12N15/113 ,  C12N1/38 ,  C12N15/85 ,  C12N15/11

CPC classification number: C12P21/00 ,  C12N1/38 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/85 ,  C12N2310/14 ,  C12N2320/00 ,  C12Y101/01027 ,  C12Y102/04001

Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.

公开(公告)号：US20180282398A1

公开(公告)日：2018-10-04

申请号：US15761887

申请日：2016-09-21

Applicant: Genentech, Inc.

Inventor： Benjamin HALEY ,  Zhilan HU ,  John C. JOLY ,  Amy Y. SHEN ,  Bradley Richard SNEDECOR

IPC: C07K16/00 ,  C12N15/113

Abstract: The present invention provides compositions comprising a Fc-containing protein wherein substantially all the Fc domains have a C-terminal lysine. Further provided are host cell for producing said compositions, methods of making said host cells and compositions, and method of use thereof.