公开(公告)号：US20240010709A1

公开(公告)日：2024-01-11

申请号：US18201904

申请日：2023-05-25

Applicant: Genentech, Inc.

Inventor： Ryan Christopher Hundley ,  Michael Wilson Laird ,  Jerome Joseph Bill ,  Christopher J. Dowd

IPC: C07K16/00 ,  C12P21/00

CPC classification number: C07K16/00 ,  C12P21/005 ,  C07K2317/14

Abstract: Methods for preparing mammalian cells for perfusion cell culture processes that improve the growth and productivity of the cells are provided. Also provided are cell culture methods that involve subjecting mammalian cells to one or more perfusion procedures, which involve generating a solids phase and a liquid phase with a continuous flow centrifuge, and utilizing at least a portion of the solids phase to sustain, maintain and/or initiate a new cell culture. Perfusion bioreactor systems and components thereof are also provided.