公开(公告)号：US20200017601A1

公开(公告)日：2020-01-16

申请号：US16353207

申请日：2019-03-14

Applicant: Genentech, Inc.

Inventor： Sunil BHAKTA ,  Hans ERICKSON ,  Jagath R. JUNUTULA ,  Katherine KOZAK ,  Rachana OHRI ,  Thomas PILLOW

IPC: C07K16/32 ,  A61K47/68 ,  C07K16/18 ,  C07K16/28 ,  C07K16/30 ,  A61K51/10 ,  C07K16/00 ,  C07K16/40 ,  G01N33/58

Abstract: Cysteine engineered antibodies comprising a free cysteine amino acid in the heavy chain or light chain are prepared by mutagenizing a nucleic acid sequence of a parent antibody and replacing one or more amino acid residues by cysteine to encode the cysteine engineered antibody; expressing the cysteine engineered antibody; and isolating the cysteine engineered antibody.

公开(公告)号：US20160130358A1

公开(公告)日：2016-05-12

申请号：US14851348

申请日：2015-09-11

Applicant: Genentech, Inc.

Inventor： Sunil BHAKTA ,  Hans ERICKSON ,  Jagath R. JUNUTULA ,  Katherine KOZAK ,  Rachana OHRI ,  Thomas PILLOW

IPC: C07K16/32 ,  C07K16/28 ,  C07K16/30 ,  G01N33/58 ,  A61K49/00 ,  A61K51/10 ,  A61K47/48 ,  C07K16/00 ,  C07K16/40

CPC classification number: C07K16/32 ,  A61K47/6803 ,  A61K47/6809 ,  A61K47/6817 ,  A61K47/6849 ,  A61K47/6869 ,  A61K51/10 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/18 ,  C07K16/28 ,  C07K16/2803 ,  C07K16/3069 ,  C07K16/3092 ,  C07K16/40 ,  C07K2317/40 ,  C07K2317/73 ,  C07K2317/94 ,  G01N33/582

Abstract: Cysteine engineered antibodies comprising a free cysteine amino acid in the heavy chain or light chain are prepared by mutagenizing a nucleic acid sequence of a parent antibody and replacing one or more amino acid residues by cysteine to encode the cysteine engineered antibody; expressing the cysteine engineered antibody; and isolating the cysteine engineered antibody.

Abstract translation: 在重链或轻链中包含游离半胱氨酸氨基酸的半胱氨酸改造的抗体是通过诱变亲本抗体的核酸序列并用半胱氨酸代替一个或多个氨基酸残基来编码半胱氨酸改造的抗体来制备的; 表达半胱氨酸改造的抗体; 并分离半胱氨酸改造的抗体。