公开(公告)号：US20180327811A1

公开(公告)日：2018-11-15

申请号：US16042320

申请日：2018-07-23

申请人： General Electric Company

发明人： Patrick McCoy SPOONER ,  Peter James TATNELL ,  Jeffrey Kenneth HORTON ,  John Richard NELSON ,  Michael John GERDES ,  Suzana KIEL ,  Ralf LENIGK ,  Alexander SCHENK ,  Wei SUN ,  Thomas HANSEN

IPC分类号： C12Q1/6804 ,  C12Q1/6869 ,  C12Q1/6876 ,  C07K16/44 ,  C12N15/11

CPC分类号： C12Q1/6804 ,  C07K16/44 ,  C12N15/11 ,  C12N2310/16 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2522/101

摘要： The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method comprises, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific binding element. This results in the protamine-specific binding element binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.