公开(公告)号：US10227645B2

公开(公告)日：2019-03-12

申请号：US15274770

申请日：2016-09-23

Applicant: Genia Technologies, Inc.

Inventor： Timothy Kellogg Craig ,  Cynthia Ann Cech ,  Michael Dorwart ,  Liv Elisabeth Jensen ,  Marshall Winston Porter ,  Christos Tzitzilonis ,  Alexander Hyun-min Yang

IPC: C07K14/31 ,  C12Q1/6869

Abstract: Described herein are engineered alpha-hemolysin subunits having mutated oligomerization domains for assembling into heptameric nanopores in lipid bilayers.

公开(公告)号：US20200216887A1

公开(公告)日：2020-07-09

申请号：US16071406

申请日：2017-01-20

Applicant: Genia Technologies, Inc. ,  ROCHE DIAGNOSTICS GMBH

Inventor： Timothy Kellogg Craig ,  Christos Tzitzilonis ,  Alexander H. Yang ,  Liv E. Jensen ,  Marshall Porter ,  Charlotte Yang ,  Corissa Harris ,  Matt Dipetro

IPC: C12Q1/6869 ,  C12N9/12

Abstract: A method is provided for preparing nanopore sequencing complexes in membranes for sequencing of polymers, e.g., polynucleotides and polypeptides. The nanopore sequencing complex is formed by the sequential linking of an enzyme to a nanopore that is inserted in a membrane, and of a polymer to the enzyme. Alternatively, the nanopore sequencing complex is formed by linking a preformed enzyme-polymer complex to a nanopore that is inserted in a membrane. The enzyme polymer complex is interchangeable.