公开(公告)号：US09605309B2

公开(公告)日：2017-03-28

申请号：US14073445

申请日：2013-11-06

Applicant: Genia Technologies, Inc.

Inventor： Randall Davis ,  Roger Chen ,  Arkadiusz Bibillo ,  Daniel Korenblum

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  G01N27/44717 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/113 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2565/631

Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.

公开(公告)号：US20190225656A1

公开(公告)日：2019-07-25

申请号：US16375140

申请日：2019-04-04

Applicant: Genia Technologies, Inc.

Inventor： Michael Dorwart ,  Daniel Korenblum

IPC: C07K14/31 ,  C12Q1/6869

Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The α-hemolysin variants have a decreased time to thread.

公开(公告)号：US10301361B2

公开(公告)日：2019-05-28

申请号：US14924861

申请日：2015-10-28

Applicant: Genia Technologies, Inc.

Inventor： Michael Dorwart ,  Daniel Korenblum

IPC: C07K14/31 ,  C12Q1/6869

Abstract: Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The α-hemolysin variants have a decreased time to thread.

公开(公告)号：US20140134616A1

公开(公告)日：2014-05-15

申请号：US14073445

申请日：2013-11-06

Applicant: Genia Technologies, Inc.

Inventor： Randall Davis ,  Roger Chen ,  Arkadiusz Bibillo ,  Daniel Korenblum ,  Michael Dorwart

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  G01N27/44717 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2527/113 ,  C12Q2535/122 ,  C12Q2563/113 ,  C12Q2565/631

Abstract: This disclosure provides chips, systems and methods for sequencing a nucleic acid sample. Tagged nucleotides are provided into a reaction chamber comprising a nanopore in a membrane. An individual tagged nucleotide of the tagged nucleotides can contain a tag coupled to a nucleotide, which tag is detectable with the aid of the nanopore. Next, an individual tagged nucleotide of the tagged nucleotides can be incorporated into a growing strand complementary to a single stranded nucleic acid molecule derived from the nucleic acid sample. With the aid of the nanopore, a tag associated with the individual tagged nucleotide can be detected upon incorporation of the individual tagged nucleotide. The tag can be detected with the aid of the nanopore when the tag is released from the nucleotide.

Abstract translation: 本公开提供了用于对核酸样品进行测序的芯片，系统和方法。 将标记的核苷酸提供到包含膜中的纳米孔的反应室中。 标记的核苷酸的单个标记的核苷酸可以含有与核苷酸偶联的标签，该标签借助于纳米孔可检测。 接下来，标记的核苷酸的单个标记的核苷酸可以掺入与衍生自核酸样品的单链核酸分子互补的生长链中。 借助纳米孔，可以在掺入单个标记的核苷酸时检测与单个标记的核苷酸相关的标签。 当标签从核苷酸释放时，可以借助纳米孔检测标签。