公开(公告)号：US20240301395A1

公开(公告)日：2024-09-12

申请号：US18653435

申请日：2024-05-02

申请人： Purigen Biosystems, Inc.

发明人： Lewis A. Marshall ,  Amy L. Hiddessen ,  Nathan P. Hoverter ,  Klint A. Rose ,  Juan G. Santiago

IPC分类号： C12N15/10 ,  B01L3/00 ,  B81B1/00 ,  B81B7/00 ,  C12Q1/6806 ,  G01N27/447

CPC分类号： C12N15/101 ,  B01L3/502753 ,  C12N15/10 ,  C12Q1/6806 ,  G01N27/4473 ,  G01N27/44739 ,  G01N27/44791 ,  G01N27/44795 ,  B01L3/502738 ,  B01L2300/0627 ,  B01L2300/0867 ,  B81B1/004 ,  B81B7/0087 ,  B81B2201/0278 ,  B81B2201/05 ,  B81B2203/0338 ,  G01N27/44704

摘要： The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.

公开(公告)号：US12078629B2

公开(公告)日：2024-09-03

申请号：US18072189

申请日：2022-11-30

申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE ,  UNIVERSITY OF MASSACHUSETTS

发明人： Lene V. Hau ,  Jene A. Golovchenko ,  Min Chen

IPC分类号： G01N33/487 ,  G01N27/447 ,  G01N33/543

CPC分类号： G01N33/48721 ,  G01N27/44791 ,  G01N33/5438

摘要： Systems and methods are provided for trapping and electrically monitoring molecules in a nanopore sensor. The nanopore sensor comprises a support structure with a first and a second fluidic chamber, at least one nanopore fluidically connected to the two chambers, and a protein shuttle. The protein shuttle comprises an electrically charged protein molecule, such as Avidin. The nanopore can be a Clytosolin A. A method can comprise applying a voltage across the nanopores to draw protein shuttles towards the nanopores. The ionic current through each or all of the nanopores can be concurrently measured. Based on the measured ionic current, blockage events can be detected. Each blockage event indicates a capture of a protein shuttle by at least one nanopore. Each blockage event can be detected through a change of the total ionic current flow or a change in the ionic current flow for a particular nanopore.

公开(公告)号：US20240264115A1

公开(公告)日：2024-08-08

申请号：US18440572

申请日：2024-02-13

申请人： ProteinSimple

发明人： Tom W. YANG ,  David John ROACH

IPC分类号： G01N27/447 ,  C07K1/26 ,  C07K1/28

CPC分类号： G01N27/44782 ,  G01N27/44704 ,  C07K1/26 ,  C07K1/285 ,  G01N27/44726 ,  G01N27/44791

摘要： An apparatus includes a body portion that defines a reservoir and a set of substantially flexible capillaries. The set of substantially flexible capillaries are fixedly coupled to the body portion and in fluid communication with the reservoir. A connector is configured to be coupled to the body portion to be in fluid communication with the reservoir and the set of substantially flexible capillaries. The connector is further configured to be coupled to a vacuum source. The apparatus is arranged such that at least a part of the body portion is electrically conductive. Methods for separating and detecting an analyte from a biological sample with the apparatus are also provided. For example, methods for separating and detecting one or more proteins from a cellular lysate or a purified protein are also provided.

公开(公告)号：US20240248061A1

公开(公告)日：2024-07-25

申请号：US18423066

申请日：2024-01-25

申请人： CytoRecovery, Inc

发明人： Alexandra Rochelle HYLER ,  Devin Monroe KECK ,  Ridi BARUA

IPC分类号： G01N27/447 ,  B03C5/00 ,  B03C5/02 ,  G01N15/12

CPC分类号： G01N27/44791 ,  B03C5/005 ,  B03C5/026 ,  G01N15/12 ,  G01N27/44769

摘要： Disclosed herein are dielectrophoresis (DEP) devices having an alignment region, a DEP region, and an analysis region for characterizing particles. Disclosed herein are methods of characterizing particles, the methods include aligning particles along one or more walls of a microfluidic device, applying a non-uniform electric field to the particles, receiving the particles in a plurality of outlet channels, respectively, based on one or more electrical properties of the plurality of particles, passing at least one of the particles in one of the plurality of outlet channels through an impedance detector, and/or detecting an impedance measurement characteristic of at least one property of the at least one of the particles.

公开(公告)号：US20240232366A1

公开(公告)日：2024-07-11

申请号：US18615758

申请日：2024-03-25

申请人： Schlumberger Technology Corporation

发明人： Simon Ivar ANDERSEN ,  Sharath Chandra Mahavadi ,  Salim TAOUTAOU ,  Alexander NEBESNYY ,  Jonathan Wun Shiung Chong

IPC分类号： G06F21/57 ,  B28C7/02 ,  B28C7/04 ,  E21B33/13 ,  G01N27/447 ,  H04L9/08 ,  H04L9/32 ,  H04L9/40

CPC分类号： G06F21/575 ,  B28C7/024 ,  B28C7/0418 ,  G01N27/44791 ,  H04L9/0825 ,  H04L9/0861 ,  H04L9/0891 ,  H04L9/321 ,  H04L9/3247 ,  H04L9/3263 ,  H04L9/3268 ,  H04L9/3297 ,  E21B33/13 ,  H04L63/08 ,  H04L63/0823 ,  H04L2463/121

摘要： A liquid additive mixing apparatus is provided that has a plurality of chambers containing additives, as well as a system for mixing the additives. One or more additives are mixed with water to form a mixing fluid. The mixing fluid is placed in a first tank that is fluidly connected to a cement mixing unit. A cementing operation is executed during which the mixing fluid from the first tank is mixed with a cement to form a slurry. A capillary electrophoresis (CE) instrument is employed to monitor at least one additive parameter and detect deviations from a predetermined tolerance for the at least one additive parameter.

公开(公告)号：US20240198343A1

公开(公告)日：2024-06-20

申请号：US18592457

申请日：2024-02-29

申请人： mirOculus Inc.

发明人： Mais J. JEBRAIL ,  Irena BARBULOVIC-NAD ,  Lorenzo GUTIERREZ ,  Foteini CHRISTODOULOU

IPC分类号： B01L3/00 ,  C12M3/06 ,  G01N27/447

CPC分类号： B01L3/502792 ,  C12M23/16 ,  G01N27/44791 ,  B01L2200/027 ,  B01L2200/142 ,  B01L2300/042 ,  B01L2300/0874 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0427

摘要： Air-matrix digital microfluidics (DMF) apparatuses and methods of using them to prevent or limit evaporation and surface fouling of the DMF apparatus. In particular, described herein are air-matrix DMF apparatuses and methods of using them in which a separate well that is accessible from the air gap of the DMF apparatus isolates a reaction droplet by including a cover to prevent evaporation. The cover may be a lid or cap, or it may be an oil or wax material within the well. The opening into the well and/or the well itself may include actuation electrodes to allow the droplet to be placed into, and in some cases removed from, the well. Also described herein are air-matrix DMF apparatuses and methods of using them including thermally controllable regions with a wax material that may be used to selectively encapsulate a reaction droplet in the air gap of the apparatus.

公开(公告)号：US20240191283A1

公开(公告)日：2024-06-13

申请号：US18348235

申请日：2023-07-06

申请人： Roche Sequencing Solutions, Inc.

发明人： Robert M. McRuer ,  Mark Stamatios Kokoris

IPC分类号： C12Q1/6825 ,  C12Q1/6869 ,  G01N27/447 ,  G01N33/487

CPC分类号： C12Q1/6825 ,  C12Q1/6869 ,  G01N27/44704 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/631

摘要： Translocation control for sensing by a nanopore, as well as methods and products related to the same, are provided. Such methods optimize duplex stability to provide high fill rate (of the hybridization sites) but do not prevent rapid dissociation required for high read rates, as well as controlling the translocation of a target molecule for sensing by a nanopore by use of a selective pulsed voltage. Products related to the same include a reporter construct comprising two or more phosphoramidites.

公开(公告)号：US12006496B2

公开(公告)日：2024-06-11

申请号：US18141641

申请日：2023-05-01

申请人： Purigen Biosystems, Inc.

发明人： Lewis A. Marshall ,  Amy L. Hiddessen ,  Nathan P. Hoverter ,  Klint A. Rose ,  Juan G. Santiago

IPC分类号： G01N27/453 ,  B01L3/00 ,  C12N15/10 ,  C12Q1/6806 ,  G01N27/447 ,  B81B1/00 ,  B81B7/00

CPC分类号： C12N15/101 ,  B01L3/502753 ,  C12N15/10 ,  C12Q1/6806 ,  G01N27/4473 ,  G01N27/44739 ,  G01N27/44791 ,  G01N27/44795 ,  B01L3/502738 ,  B01L2300/0627 ,  B01L2300/0867 ,  B81B1/004 ,  B81B7/0087 ,  B81B2201/0278 ,  B81B2201/05 ,  B81B2203/0338 ,  G01N27/44704 ,  C12N15/101 ,  C12Q2565/125

摘要： The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more analytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing.

公开(公告)号：US11988633B2

公开(公告)日：2024-05-21

申请号：US16644467

申请日：2018-09-20

申请人： Agilent Technologies, Inc.

发明人： Fielito U. Limjap ,  Michael J. Kimzey ,  Francis T. Haxo

IPC分类号： G01N27/447 ,  C08B37/00 ,  C08G73/02 ,  C08L79/02

CPC分类号： G01N27/44747 ,  C08B37/0003 ,  C08B37/0087 ,  C08G73/0206 ,  C08G73/0273 ,  C08L79/02 ,  G01N27/44791

摘要： The present disclosure provides methods of improving the resolution of labeled glycans in capillary electrophoresis techniques using a gel as a sieving matrix, by incorporating polyamines in the gel.

公开(公告)号：US11953494B2

公开(公告)日：2024-04-09

申请号：US17457843

申请日：2021-12-06

申请人： Roche Sequencing Solutions, Inc.

发明人： Yuri Mitnick ,  Xu Ouyang ,  Janusz B. Wojtowicz

IPC分类号： G01N33/487 ,  B01L3/00 ,  C12Q1/6869 ,  G01N27/447

CPC分类号： G01N33/48721 ,  B01L3/502715 ,  C12Q1/6869 ,  G01N27/44791 ,  B01L2200/027 ,  B01L2300/0636

摘要： A nanopore-based sequencing system includes a plurality of nanopore-based sequencing chips. Each of the nanopore-based sequencing chips comprises a plurality of nanopore sensors. The system comprises at least one flow cell coupled to at least one of the plurality of nanopore-based sequencing chips, wherein the flow cell coupled to the at least one of the plurality of nanopore-based sequencing chips comprises one or more fluidic flow channels that allow a fluid external to the system to flow on top of the nanopore-based sequencing chip and out of the system. The system further comprises a printed circuit board electrically connected to the plurality of nanopore-based sequencing chips.