公开(公告)号：US20180340157A1

公开(公告)日：2018-11-29

申请号：US15989109

申请日：2018-05-24

Applicant: Genia Technologies, Inc.

Inventor： Helen FRANKLIN ,  Cynthia CECH ,  Timothy Kellogg CRAIG ,  Aruna AYER ,  Kirti DHIMAN ,  Natalie B. CHECHELSKI JOHNSTON ,  Joshua N. MABRY ,  Arkadiusz BIBILLO ,  Peter CRISALLI ,  Randall W. DAVIS

IPC: C12N9/12

Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.