公开(公告)号：US20150152494A1

公开(公告)日：2015-06-04

申请号：US14521425

申请日：2014-10-22

Applicant: Genia Technologies, Inc.

Inventor： Randall W. DAVIS ,  Edward Shian LIU ,  Eric Takeshi HARADA ,  Anne AGUIRRE ,  Andrew TRANS ,  James POLLARD ,  Cynthia CECH

IPC: C12Q1/68 ,  G01N27/327

CPC classification number: C12Q1/6869 ,  C12M1/34 ,  G01N27/3277 ,  G01N27/3278 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.

Abstract translation: 本公开提供了包括多个孔的生物芯片。 生物芯片包括设置在多个孔的单个井中或邻近多个井的单个井的膜。 膜包括纳米孔，并且单个孔包括电极，其响应于通过或邻近纳米孔的物质而在离子流动通过孔时检测信号。 电极可以是非牺牲电极。 可以使用气泡在多个孔上形成脂质双层。

公开(公告)号：US20180362594A1

公开(公告)日：2018-12-20

申请号：US15762092

申请日：2016-09-20

Applicant: GENIA TECHNOLOGIES, INC.

Inventor： Cynthia CECH ,  Tim CRAIG ,  Christos TZITZILONIS ,  Alexander YANG ,  Liv JENSEN ,  Charlotte YANG ,  Corissa HARRIS

IPC: C07K14/245 ,  C12N9/12 ,  C12Q1/6874

CPC classification number: C07K14/245 ,  C07K2319/00 ,  C12N9/1252 ,  C12Q1/68 ,  C12Q1/6874 ,  C12Y207/07007 ,  G01N33/6803 ,  C12Q2525/197 ,  C12Q2525/203 ,  C12Q2565/631

Abstract: The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

公开(公告)号：US20190185927A1

公开(公告)日：2019-06-20

申请号：US16272970

申请日：2019-02-11

Applicant: Genia Technologies, Inc.

Inventor： Randall W. DAVIS ,  Edward Shian LIU ,  Eric Takeshi HARADA ,  Anne AGUIRRE ,  Andrew TRANS ,  James POLLARD ,  Cynthia CECH

IPC: C12Q1/6869 ,  G01N27/447 ,  G01N27/327 ,  C12M1/34 ,  G01N33/487

CPC classification number: C12Q1/6869 ,  C12M1/34 ,  G01N27/3277 ,  G01N27/3278 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.

公开(公告)号：US20180340157A1

公开(公告)日：2018-11-29

申请号：US15989109

申请日：2018-05-24

Applicant: Genia Technologies, Inc.

Inventor： Helen FRANKLIN ,  Cynthia CECH ,  Timothy Kellogg CRAIG ,  Aruna AYER ,  Kirti DHIMAN ,  Natalie B. CHECHELSKI JOHNSTON ,  Joshua N. MABRY ,  Arkadiusz BIBILLO ,  Peter CRISALLI ,  Randall W. DAVIS

IPC: C12N9/12

Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.

公开(公告)号：US20150153302A1

公开(公告)日：2015-06-04

申请号：US14521427

申请日：2014-10-22

Applicant: Genia Technologies, Inc.

Inventor： Randall W. DAVIS ,  Edward Shian LIU ,  Eric Takeshi HARADA ,  Anne AGUIRRE ,  Andrew TRANS ,  James POLLARD ,  Cynthia CECH

IPC: G01N27/327

CPC classification number: C12Q1/6869 ,  C12M1/34 ,  G01N27/3277 ,  G01N27/3278 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.

Abstract translation: 公开了用于分子检测和感测的生物芯片。 生物芯片包括基底。 生物芯片包括形成在衬底上的多个离散位置，其密度大于每平方毫米500个孔。 每个离散部位包括设置在基底上以形成孔的侧壁。 每个离散位置包括设置在井的底部的电极。 在一些实施例中，形成井，使得井之间的串扰减少。 在一些实施例中，设置在阱的底部的电极被组织成电极组，其中每组电极共享公共对电极。 在一些实施例中，设置在阱底部的电极具有专用的对电极。 在一些实施例中，侧壁的表面被硅烷化，使得表面有助于在孔中或邻近孔的膜的形成。

公开(公告)号：US20170198343A1

公开(公告)日：2017-07-13

申请号：US15385779

申请日：2016-12-20

Applicant: Genia Technologies, Inc.

Inventor： Randall W. DAVIS ,  Edward Shian LIU ,  Eric Takeshi HARADA ,  Anne AGUIRRE ,  Andrew TRANS ,  James POLLARD ,  Cynthia CECH

IPC: C12Q1/68 ,  G01N27/327 ,  G01N27/447 ,  G01N33/487

CPC classification number: C12Q1/6869 ,  C12M1/34 ,  G01N27/3277 ,  G01N27/3278 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.

公开(公告)号：US20160327507A1

公开(公告)日：2016-11-10

申请号：US15066117

申请日：2016-03-10

Applicant: Genia Technologies, Inc.

Inventor： Randall W. DAVIS ,  Edward Shian LIU ,  Eric Takeshi HARADA ,  Anne AGUIRRE ,  Andrew TRANS ,  James POLLARD ,  Cynthia CECH ,  Hui TIAN ,  Robert YUAN ,  John FOSTER ,  Roger CHEN

IPC: G01N27/327 ,  C12Q1/68 ,  G01N33/487

CPC classification number: C12Q1/6869 ,  C12M1/34 ,  G01N27/3277 ,  G01N27/3278 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.