公开(公告)号：US20070020636A1

公开(公告)日：2007-01-25

申请号：US10576005

申请日：2006-03-28

申请人： Georg Sczakiel ,  Valentin Vlassov ,  Pavel Laktionov ,  Elena Rykova ,  Svetlana Tamkovic ,  Tat'yana Skvortsova

发明人： Georg Sczakiel ,  Valentin Vlassov ,  Pavel Laktionov ,  Elena Rykova ,  Svetlana Tamkovic ,  Tat'yana Skvortsova

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6806 ,  C12Q1/6886 ,  C12Q2527/125

摘要： This invention relates to noninvasive methods of early detection of different sicknesses, like precancerous state or early stages of cancer development, pathologies of pregnancy, and monitoring of efficacy of anticancer therapy. The method is based on cell-surface extra-cellular nucleic acids from human blood which is divided into plasma and cellular fractions, and further divided into Leukocytes and erythrocytes. Cell-Surface-bound extra-cellular nucleic acids are eluted form cell surface with PBS-EDTA treatment or treatment of cells with trypsin solution. Eluted nucleic acids are isolated with convenient method and analyzed for presence of at least two specific sequences of nucleic acids such as PCR analysis, multiplex PCR, hybridization assay or other methods, thereby increasing the reliability of early detection of the diseases with abnormal functioning of genetic apparatus of cells due to increase of sensitivity of detection of specific DNA and RNA sequences.

摘要翻译： 本发明涉及早期检测不同疾病的非侵入性方法，如癌症发展的癌前状态或早期阶段，妊娠病理学以及抗癌治疗功效的监测。 该方法基于来自人血液的细胞表面细胞外核酸，其被分为血浆和细胞级分，并进一步分为白细胞和红细胞。 用PBS-EDTA处理或用胰蛋白酶溶液处理细胞从细胞表面洗脱细胞表面结合的细胞外核酸。 用方便的方法分离洗脱的核酸，并分析至少两种核酸特异性序列的存在，如PCR分析，多重PCR，杂交测定或其他方法，从而增加早期检测遗传异常功能的疾病的可靠性 由于检测特异性DNA和RNA序列的灵敏度增加，细胞的装置。