公开(公告)号：US20110076694A1

公开(公告)日：2011-03-31

申请号：US12920670

申请日：2009-03-05

申请人： Gerald M. Kidder ,  Hong-Xing Wang ,  Dan Tong ,  Francis R. Tekpetey

发明人： Gerald M. Kidder ,  Hong-Xing Wang ,  Dan Tong ,  Francis R. Tekpetey

IPC分类号： G01N33/53 ,  C12Q1/02

CPC分类号： G01N33/56966 ,  G01N33/5091 ,  G01N2333/705

摘要： A method of assessing the health of each oocyte or embryo of a mammal from a plurality of oocytes/embryos of the mammal is provided. The method comprises the steps of: measuring gap junctional coupling strength or connexin43 expression of follicular cells associated with each oocyte; and identifying the gap junctional coupling strength/connexin43 expression of follicular cells associated with each oocyte, wherein the greater the coupling strength/connexin43 expression of follicular cells associated with an oocyte relative to the coupling strength/connexin43 expression of follicular cells associated with each other oocyte, the healthier the oocyte and the embryo resulting from that oocyte. Kits for conducting these methods are also provided.

摘要翻译： 提供了一种从哺乳动物的多个卵母细胞/胚胎评估哺乳动物的每个卵母细胞或胚胎的健康状况的方法。 该方法包括以下步骤：测量与每个卵母细胞相关的滤泡细胞的间隙连接耦合强度或连接蛋白43表达; 并鉴定与每个卵母细胞相关的滤泡细胞的间隙连接耦合强度/连接蛋白43表达，其中与卵母细胞相关的滤泡细胞的耦合强度/连接蛋白43表达相对于与彼此卵母细胞相关的滤泡细胞的偶联强度/连接蛋白43表达越大 ，由卵母细胞产生的卵母细胞和胚胎更健康。 还提供了用于进行这些方法的套件。