公开(公告)号：US20110045531A1

公开(公告)日：2011-02-24

申请号：US12599843

申请日：2008-05-16

申请人： Gerald Striedner ,  Johann Huber ,  Daniela Keller

发明人： Gerald Striedner ,  Johann Huber ,  Daniela Keller

IPC分类号： C12P21/00

CPC分类号： C12P21/02 ,  C12N15/70 ,  C12N15/902

摘要： A method for producing a protein of interest on a manufacturing scale is based on integration, by homologous recombination, of the DNA encoding the protein of interest into a bacterial cell genome at a pre-selected site. The manufacturing scale production of recombinant proteins is in the fed-batch mode, semi-continuous or in a chemostat.

摘要翻译： 在制造规模上生产感兴趣的蛋白质的方法是基于通过同源重组将编码目标蛋白质的DNA整合到预先选择的位点处的细菌细胞基因组中。 重组蛋白质的制造规模生产以补料分批模式，半连续或恒化器。

公开(公告)号：US09683252B2

公开(公告)日：2017-06-20

申请号：US12599843

申请日：2008-05-16

申请人： Gerald Striedner ,  Johann Huber ,  Daniela Keller

发明人： Gerald Striedner ,  Johann Huber ,  Daniela Keller

IPC分类号： C12P21/02 ,  C12N15/70 ,  C12N15/90

CPC分类号： C12P21/02 ,  C12N15/70 ,  C12N15/902

摘要： A method for producing a protein of interest on a manufacturing scale is based on integration, by homologous recombination, of the DNA encoding the protein of interest into a bacterial cell genome at a pre-selected site. The manufacturing scale production of recombinant proteins is in the fed-batch mode, semi-continuous or in a chemostat.