公开(公告)号：US20090117532A1

公开(公告)日：2009-05-07

申请号：US12254151

申请日：2008-10-20

申请人： Gerald V. DOYLE

发明人： Gerald V. DOYLE

IPC分类号： A01N1/02

CPC分类号： G01N33/54326 ,  G01N33/57415

摘要： The CellTracks® System provides a system to enumerate CTC's in blood. The system immunomagnetically concentrates epithelial cells, fluorescently labels the cells and identifies and quantifies CTC's. The absolute number of CTC's detected in the peripheral blood tumor load is, in part, a factor in prediction of survival, time to progression, and response to therapy. Pre-clinical studies of circulating tumor cells (CTC's) have been limited by the inability to repetitively monitor CTC's in animal models. The present invention provides a method to enumerate CTC's in blood samples obtained from living mice, using a protocol similar to an in vitro diagnostic system for quantifying CTC's in patients. Accordingly, this technology can be adapted for serial monitoring of CTC's in mouse xenograft tumor models of human breast cancer.

摘要翻译： CellTracks（R）系统提供一个系统来列举血液中的四氯化碳。 该系统免疫磁性浓缩上皮细胞，荧光标记细胞，并识别和量化CTC。 在外周血肿瘤负荷中检测到的四氯化碳的绝对数量部分是预测生存期，进展时间和治疗反应的因素。 循环肿瘤细胞（CTC）的临床前研究受到动物模型中不能重复监测CTC的限制。 本发明提供了一种使用类似于用于定量患者中的CTC的体外诊断系统的方案来列举从活小鼠获得的血液样品中的CTC的方法。 因此，该技术可适用于人乳腺癌小鼠异种移植肿瘤模型中CTC的连续监测。