公开(公告)号：US07593649B1

公开(公告)日：2009-09-22

申请号：US10935517

申请日：2004-09-07

申请人： Gerard Talatinian ,  Daniel Pierce

发明人： Gerard Talatinian ,  Daniel Pierce

IPC分类号： H04B10/06

CPC分类号： H04B10/1141

摘要： Method and system for processing a modulated WIR input signal. A digital device includes an input receiving circuit. This circuit receives the modulated WIR input signal and detects a modulation carrier pulse burst in the WIR input signal and generates a valid pulse signal. A pulse generating circuit is operatively coupled to the input receiving circuit and generates a pulse signal of a specific duration based in part on the pulse burst.

摘要翻译： 用于处理调制WIR输入信号的方法和系统。 数字装置包括输入接收电路。 该电路接收调制的WIR输入信号，并检测WIR输入信号中的调制载波脉冲串，并产生有效的脉冲信号。 脉冲发生电路可操作地耦合到输入接收电路，并且部分地基于脉冲串产生特定持续时间的脉冲信号。

公开(公告)号：US09103282B2

公开(公告)日：2015-08-11

申请号：US13277630

申请日：2011-10-20

申请人： Richard Charron ,  Daniel Pierce

发明人： Richard Charron ,  Daniel Pierce

IPC分类号： F01D5/08 ,  F01D25/08 ,  F02C7/12 ,  F02C7/20 ,  F02C7/36

CPC分类号： F02C7/12 ,  F01D5/08 ,  F01D25/08 ,  F02C7/20 ,  F02C7/36

摘要： A shaft cover support for a gas turbine engine is disclosed. The shaft cover support not only provides enhanced support to a shaft cover of the gas turbine engine, but also includes a cooling fluid chamber for passing fluids from a rotor air cooling supply conduit to an inner ring cooling manifold. Furthermore, the shaft cover support may include a cooling shield supply extending from the cooling fluid chamber between the radially outward inlet and the radially inward outlet on the radially extending region and in fluid communication with the cooling fluid chamber for providing cooling fluids to a transition section. The shaft cover support may also provide additional stiffness and reduce interference of the flow from the compressor. In addition, the shaft cover support accommodates a transition section extending between compressor and turbine sections of the gas turbine engine.

摘要翻译： 公开了一种用于燃气涡轮发动机的轴盖支撑件。 轴盖支撑件不仅对燃气涡轮发动机的轴盖提供增强的支撑，还包括用于将流体从转子空气冷却供应管道传递到内环冷却歧管的冷却流体室。 此外，轴盖支撑件可以包括冷却屏蔽供应源，其从径向向外的入口和径向向外的出口之间的冷却流体室延伸并且与冷却流体室流体连通，以将冷却流体提供给过渡部分 。 轴盖支撑件还可以提供额外的刚度并且减小来自压缩机的流动的干扰。 此外，轴套支撑件容纳在燃气涡轮发动机的压缩机和涡轮机部分之间延伸的过渡部分。

公开(公告)号：US20080096225A1

公开(公告)日：2008-04-24

申请号：US11881311

申请日：2007-07-25

申请人： Ronald Vale ,  Daniel Pierce ,  James Spudich ,  Lawrence Goldstein

发明人： Ronald Vale ,  Daniel Pierce ,  James Spudich ,  Lawrence Goldstein

IPC分类号： G01N33/53

CPC分类号： C12Q1/42 ,  C12Q1/34 ,  G01N33/68 ,  G01N33/6842 ,  G01N2333/914 ,  G01N2500/00 ,  G01N2500/02

摘要： Described herein are methods of identifying compounds which modulate the activity of the cytoskeletal system. The methods are rapid, convenient and sensitive. Preferably, the method is used to identify lead compounds that can be used as therapeutics, diagnostics and agricultural agents. Generally, test compounds are added to two cytoskeletal components which bind to one another, to determine whether the binding is affected by the test compound. Wherein the binding is affected, a compound which modulates the cytoskeletal system is identified.

摘要翻译： 本文描述了鉴定调节细胞骨架系统的活性的化合物的方法。 方法快速，方便，灵敏。 优选地，该方法用于鉴定可用作治疗剂，诊断剂和农药的铅化合物。 通常，将测试化合物加入到彼此结合的两个细胞骨架组分中，以确定结合是否受到测试化合物的影响。 其中结合受到影响，鉴定出调节细胞骨架系统的化合物。

公开(公告)号：US07252950B1

公开(公告)日：2007-08-07

申请号：US10031819

申请日：1998-09-03

申请人： Ronald Vale ,  Daniel Pierce ,  James Spudich

发明人： Ronald Vale ,  Daniel Pierce ,  James Spudich

IPC分类号： G01N33/53 ,  C12N9/14 ,  C12N9/16

CPC分类号： C12Q1/42 ,  C12Q1/34 ,  G01N33/68 ,  G01N33/6842 ,  G01N2333/914 ,  G01N2500/00 ,  G01N2500/02

摘要： Described herein are methods of identifying compounds which modulate the activity of the cytoskeletal system. The methods are rapid, convenient and sensitive. Preferably, the method is used to identify lead compounds that can be used as therapeutics, diagnostics and agricultural agents. Generally, test compounds are added to two cytoskeletal components which bind to one another, to determine whether the binding is affected by the test compound. Wherein the binding is affected, a compound which modulates the cytoskeletal system is identified.

摘要翻译： 本文描述了鉴定调节细胞骨架系统的活性的化合物的方法。 方法快速，方便，灵敏。 优选地，该方法用于鉴定可用作治疗剂，诊断剂和农药的铅化合物。 通常，将测试化合物加入到彼此结合的两个细胞骨架组分中，以确定结合是否受到测试化合物的影响。 其中结合受到影响，鉴定出调节细胞骨架系统的化合物。

公开(公告)号：US20060257962A1

公开(公告)日：2006-11-16

申请号：US11296193

申请日：2005-12-06

申请人： Ramesh Baliga ,  Donglin Guo ,  Daniel Pierce ,  Jeffrey Finer

发明人： Ramesh Baliga ,  Donglin Guo ,  Daniel Pierce ,  Jeffrey Finer

IPC分类号： C12Q1/42

CPC分类号： G01N33/58 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/573 ,  G01N33/68 ,  G01N33/6845

摘要： The present invention provides assays for detecting ADP, GDP and inorganic phosphate. These assays can be used directly to detect the presence of ADP, GDP and inorganic phosphate or can be used as part of a number of methods for identifying candidate agents that bind to a target protein or serve as modulators of the biological activity of a target protein.

摘要翻译： 本发明提供检测ADP，GDP和无机磷酸盐的测定。 这些测定可以直接用于检测ADP，GDP和无机磷酸盐的存在，或者可以用作多种用于鉴定结合靶蛋白的候选试剂或用作靶蛋白的生物活性的调节剂的多种方法的一部分 。

公开(公告)号：US20130098065A1

公开(公告)日：2013-04-25

申请号：US13277630

申请日：2011-10-20

申请人： RICHARD CHARRON ,  Daniel Pierce

发明人： RICHARD CHARRON ,  Daniel Pierce

IPC分类号： F02C7/20 ,  F02C7/12

CPC分类号： F02C7/12 ,  F01D5/08 ,  F01D25/08 ,  F02C7/20 ,  F02C7/36

摘要： A shaft cover support for a gas turbine engine is disclosed. The shaft cover support not only provides enhanced support to a shaft cover of the gas turbine engine, but also includes a cooling fluid chamber for passing fluids from a rotor air cooling supply conduit to an inner ring cooling manifold. Furthermore, the shaft cover support may include a cooling shield supply extending from the cooling fluid chamber between the radially outward inlet and the radially inward outlet on the radially extending region and in fluid communication with the cooling fluid chamber for providing cooling fluids to a transition section. The shaft cover support may also provide additional stiffness and reduce interference of the flow from the compressor. In addition, the shaft cover support accommodates a transition section extending between compressor and turbine sections of the gas turbine engine.

摘要翻译： 公开了一种用于燃气涡轮发动机的轴盖支撑件。 轴盖支撑件不仅对燃气涡轮发动机的轴盖提供增强的支撑，还包括用于将流体从转子空气冷却供应管道传递到内环冷却歧管的冷却流体室。 此外，轴盖支撑件可以包括冷却屏蔽供应源，其从径向向外的入口和径向向外的出口之间的冷却流体室延伸并且与冷却流体室流体连通，以将冷却流体提供给过渡部分 。 轴盖支撑件还可以提供额外的刚度并且减小来自压缩机的流动的干扰。 此外，轴套支撑件容纳在燃气涡轮发动机的压缩机和涡轮机部分之间延伸的过渡部分。

公开(公告)号：US20130098064A1

公开(公告)日：2013-04-25

申请号：US13277579

申请日：2011-10-20

申请人： Richard Charron ,  Daniel Pierce

发明人： Richard Charron ,  Daniel Pierce

IPC分类号： F02C7/12 ,  F02C7/20

CPC分类号： F02C7/12 ,  F02C7/20 ,  F02C7/36

摘要： A shaft cover support for a gas turbine engine is disclosed. The shaft cover support not only provides enhanced support to a shaft cover of the gas turbine engine, but also includes a cooling fluid chamber for passing fluids from a rotor air cooling supply conduit to an inner ring cooling manifold. As such, the shaft cover support accomplishes in a single component what was only partially accomplished in two components in conventional configurations. The shaft cover support may also provide additional stiffness and reduce interference of the flow from the compressor. In addition, the shaft cover support accommodates a transition section extending between compressor and turbine sections of the engine. The shaft cover support has a radially extending region that is offset from the inlet and outlet that enables the shaft cover support to surround the transition, thereby reducing the overall length of this section of the engine.

摘要翻译： 公开了一种用于燃气涡轮发动机的轴盖支撑件。 轴盖支撑件不仅对燃气涡轮发动机的轴盖提供增强的支撑，还包括用于将流体从转子空气冷却供应管道传递到内环冷却歧管的冷却流体室。 因此，轴盖支撑件在单个部件中实现了在常规构造中仅部分实现的两个部件。 轴盖支撑件还可以提供额外的刚度并且减小来自压缩机的流动的干扰。 此外，轴盖支撑件容纳在发动机的压缩机和涡轮机部分之间延伸的过渡部分。 轴盖支撑件具有径向延伸的区域，其从入口和出口偏移，使得轴盖支撑件能够围绕过渡，从而减小发动机的该部分的总长度。

公开(公告)号：US20060003399A1

公开(公告)日：2006-01-05

申请号：US11150845

申请日：2005-06-10

申请人： Nenad Tomasevic ,  Zhiheng Jia ,  Roman Sakowicz ,  Daniel Pierce ,  Jeffrey Finer

发明人： Nenad Tomasevic ,  Zhiheng Jia ,  Roman Sakowicz ,  Daniel Pierce ,  Jeffrey Finer

IPC分类号： C12Q1/37

CPC分类号： G01N33/542 ,  G01N33/582 ,  G01N33/6887 ,  G01N2500/00

摘要： Methods for assaying for actin polymerization are described, as are the use of the assays to screen for modulators of actin polymerization. The screening methods can be utilized to identify active agents that interact with actin, the polymerization state of actin and other proteins or cellular components whose function is naturally or artificially coupled to actin polymerization or depolymerization. Protein constructs and kits useful in performing the methods are also provided.

摘要翻译： 描述了用于测定肌动蛋白聚合的方法，以及测定法用于筛选肌动蛋白聚合的调节剂的方法。 筛选方法可以用于鉴定与肌动蛋白相互作用的活性剂，肌动蛋白的聚合状态和功能是天然或人工偶联到肌动蛋白聚合或解聚的其它蛋白质或细胞组分。 还提供了可用于实施该方法的蛋白质构建体和试剂盒。

公开(公告)号：US08961118B2

公开(公告)日：2015-02-24

申请号：US13277579

申请日：2011-10-20

申请人： Richard Charron ,  Daniel Pierce

发明人： Richard Charron ,  Daniel Pierce

IPC分类号： F01D5/08 ,  F01D25/08 ,  F02C7/12 ,  F02C7/20 ,  F02C7/36

CPC分类号： F02C7/12 ,  F02C7/20 ,  F02C7/36

摘要： A shaft cover support for a gas turbine engine is disclosed. The shaft cover support not only provides enhanced support to a shaft cover of the gas turbine engine, but also includes a cooling fluid chamber for passing fluids from a rotor air cooling supply conduit to an inner ring cooling manifold. As such, the shaft cover support accomplishes in a single component what was only partially accomplished in two components in conventional configurations. The shaft cover support may also provide additional stiffness and reduce interference of the flow from the compressor. In addition, the shaft cover support accommodates a transition section extending between compressor and turbine sections of the engine. The shaft cover support has a radially extending region that is offset from the inlet and outlet that enables the shaft cover support to surround the transition, thereby reducing the overall length of this section of the engine.

摘要翻译： 公开了一种用于燃气涡轮发动机的轴盖支撑件。 轴盖支撑件不仅对燃气涡轮发动机的轴盖提供增强的支撑，还包括用于将流体从转子空气冷却供应管道传递到内环冷却歧管的冷却流体室。 因此，轴盖支撑件在单个部件中实现了在常规构造中仅部分实现的两个部件。 轴盖支撑件还可以提供额外的刚度并且减小来自压缩机的流动的干扰。 此外，轴盖支撑件容纳在发动机的压缩机和涡轮机部分之间延伸的过渡部分。 轴盖支撑件具有径向延伸的区域，其从入口和出口偏移，使得轴盖支撑件能够围绕过渡，从而减小发动机的该部分的总长度。