公开(公告)号：US06905876B2

公开(公告)日：2005-06-14

申请号：US09998573

申请日：2001-11-16

申请人： Ghulam Nabi Qazi ,  Surrinder Kumar Lattoo ,  Avtar Krishan Dhar ,  Paresh Purohit ,  Ravinder Kumar Raina ,  Rekha Sapru Dhar

发明人： Ghulam Nabi Qazi ,  Surrinder Kumar Lattoo ,  Avtar Krishan Dhar ,  Paresh Purohit ,  Ravinder Kumar Raina ,  Rekha Sapru Dhar

IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/04

CPC分类号： C12N5/0025 ,  C12N5/04

摘要： The present disclosure relates to methods and compositions for in vitro cultivation of species of Polygonatum, e.g. Polygonatum cirrhifolium Royle. The disclosure provides culture media comprising MS basal culture media and plant hormones, preferably selected from the group consisting of gibberellic acid (GA3), 6-benzyl-aminopurine (BAP), and naphthalene acetic acid (NAA). The disclosure provides methods of in vitro cultivation of Polygonatum comprising contacting Polygonatum seeds with a first medium comprising MS basal culture medium and GA3, upon emergence of a hypocotyl, transferring this primary explant to a second medium comprising MS basal culture medium, BAP, and NAA, and upon emergence of a first foliage leaf, transferring this secondary explant to a third medium comprising MS basal culture medium, BAP, NAA, and gibberellic acid (GA3). The methods and compositions of the disclosure are capable of inducing and/or supporting uniform germination in less than about 90 days, synchronized development of epicotyl, coleoptile, and radicle, termination of epicotyl dormancy and combinations thereof. The present disclosure relates to the novel culture medium compositions, said compositions comprising Murashige and Skoog (MS), a basal culture medium, varied concentrations of plant hormones, and other additives, leading to extraordinarily fast and synchronized in vitro induction of germination and release of epicotyl dormancy in Polygonatum cirrhifolium Royle, an endangered medicinal plant species and a method for faster in vitro propagation of Polygonatum cirrhifolium Royle.

摘要翻译： 本公开涉及用于体外培养Poly属物种的方法和组合物，例如， 茯苓 本公开提供了包含MS基础培养基和植物激素的培养基，优选选自赤霉酸（GA 3 N 3），6-苄基 - 氨基嘌呤（BAP）和萘乙酸（NAA ）。 本公开内容提供了Polygonatum的体外培养方法，包括在出现下胚轴的情况下将Polygonatum种子与包含MS基础培养基和GA 3的第一培养基接触，将该初级外植体转移到包含MS的第二培养基 基础培养基，BAP和NAA，并且在出现第一叶叶时，将该次级外植体转移到包含MS基础培养基，BAP，NAA和赤霉酸（GA 3 N 3）的第三培养基中， 。 本公开的方法和组合物能够在少于约90天内诱导和/或支持均匀的发芽，同时发育上胚轴，胚芽鞘和胚根，终止上胚轴休眠及其组合。 本公开涉及新型培养基组合物，所述组合物包含Murashige和Skoog（MS），基础培养基，不同浓度的植物激素和其它添加剂，导致异常快速和同步的体外诱导萌发和释放 Poly in ium。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。