公开(公告)号：US08715739B2

公开(公告)日：2014-05-06

申请号：US11552324

申请日：2006-10-24

申请人： Gil U. Lee ,  Hao Shang ,  Won-Suk Chang

发明人： Gil U. Lee ,  Hao Shang ,  Won-Suk Chang

IPC分类号： A61K9/50

CPC分类号： B01J2/06 ,  A61K41/0028 ,  A61K41/0052 ,  A61K49/1887 ,  B01J13/12 ,  B01J13/14 ,  B82Y5/00 ,  B82Y25/00 ,  H01F1/0054

摘要： Methods for preparing uniformly sized micropanicles, with an optional polymeric coating generally include: 1) providing nanoparticles, preferably having a size of between 1 nm and 100 nm; 2) adding a hydrophobic surface layer to the nanoparticles; 3) making a suspension of the hydrophobic nanoparticles and a polymerization initiator in an hydrophobic solvent; 4) dissolving a monomer in the hydrophobic solvent; 5) making an emulsion by dispersing droplets of the hydrophobic solvent in a continuous aqueous phase with an emulsifier; 6) sizing the first emulsion to provide a second emulsion of the same components in which the droplets are substantially uniform and between 2 and 20 um in size; 7) evaporating at least a substantial portion of the dispersed hydrophobic droplets to assemble nanoparticles to form micropanicles suspended now in the aqueous phase; 8) replacing the first surfactant with a second surfactant, which is preferably a polymerizable surfactant; 9) adding a polymerizable monomer to the aqueous phase and allowing it to adsorb into the microparticle; 10) polymerizing the monomer(s) to provide a polymer layer on the micropanicles; and 11) functionalizing the polymer surface layer erf the micropanicles with one or more polymer, nanoparticle or biological macromolecular layers.

摘要翻译： 用任选的聚合物涂层制备均匀尺寸的微粒子的方法通常包括：1）提供优选具有1nm至100nm尺寸的纳米颗粒; 2）向纳米颗粒中加入疏水表面层; 3）使疏水性纳米粒子和聚合引发剂在疏水性溶剂中的悬浮液; 4）将单体溶解在疏水溶剂中; 5）通过将疏水性溶剂的液滴与乳化剂分散在连续的水相中来制备乳液; 6）调整第一乳液的尺寸以提供相同组分的第二乳液，其中液滴基本上均匀且尺寸在2-20μm之间; 7）蒸发至少大部分分散的疏水液滴以组装纳米颗粒以形成现在悬浮于水相中的微球; 8）用优选可聚合表面活性剂的第二表面活性剂代替第一表面活性剂; 9）向水相中加入可聚合单体并使其吸附到微粒中; 10）聚合单体以在微粒上提供聚合物层; 和11）使用一种或多种聚合物，纳米颗粒或生物大分子层对聚合物表面层进行功能化。

公开(公告)号：US20070172426A1

公开(公告)日：2007-07-26

申请号：US11552324

申请日：2006-10-24

申请人： Gil Lee ,  Hao Shang ,  Won-Suk Chang

发明人： Gil Lee ,  Hao Shang ,  Won-Suk Chang

IPC分类号： A61K49/10 ,  B05D3/00

CPC分类号： B01J2/06 ,  A61K41/0028 ,  A61K41/0052 ,  A61K49/1887 ,  B01J13/12 ,  B01J13/14 ,  B82Y5/00 ,  B82Y25/00 ,  H01F1/0054

摘要： Methods for preparing uniformly sized microparticles, with an optional polymeric coating generally include: 1) providing nanoparticles, preferably having a size of between 1 nm and 100 nm; 2) adding a hydrophobic surface layer to the nanoparticles; 3) making a suspension of the hydrophobic nanoparticles and a polymerization initiator in an hydrophobic solvent; 4) dissolving a monomer in the hydrophobic solvent; 5) making an emulsion by dispersing droplets of the hydrophobic solvent in a continuous aqueous phase with an emulsifier; 6) sizing the first emulsion to provide a second emulsion of the same basic components but in which the droplets are substantially uniform and between 2 and 20 μm in size; 7) evaporating at least a substantial portion of the dispersed hydrophobic droplets to assemble nanoparticles to form microparticles suspended now in the aqueous phase; 8) optionally replacing the first surfactant with a second surfactant, which is preferably a polymerizable surfactant; 9) optionally adding a polymerizable monomer to the aqueous phase and allowing it to adsorb into the microparticle; 10) polymerizing the monomer(s) to provide a polymer layer on the microparticles; and 11) functionalizing the polymer surface layer of the microparticles with one or more polymer, nanoparticle or biological macromolecular layers. In a related method the nanoparticles are provided with a hydrophilic surface layer, and the emulsion is made by dispersing the aqueous nanoparticles in a continuous hydrophobic phase. The microparticles have a polymeric coating and a magnetic core that comprises in excess of 50% of the particle's mass. The polymeric shell may contain one or more functional groups capable of forming chemical bonds useful in managing a variety of separations.

摘要翻译： 用任选的聚合物涂层制备均匀尺寸的微粒的方法通常包括：1）提供优选具有1nm至100nm尺寸的纳米颗粒; 2）向纳米颗粒中加入疏水表面层; 3）使疏水性纳米粒子和聚合引发剂在疏水性溶剂中的悬浮液; 4）将单体溶解在疏水溶剂中; 5）通过将疏水性溶剂的液滴与乳化剂分散在连续的水相中来制备乳液; 6）调整第一乳液的尺寸以提供相同基本组分的第二乳液，但其中液滴基本均匀且尺寸在2至20μm之间; 7）蒸发至少大部分分散的疏水液滴以组装纳米颗粒以形成现在悬浮在水相中的微粒; 8）任选地用第二表面活性剂替代第一表面活性剂，第二表面活性剂优选为可聚合表面活 9）任选地将可聚合单体加入到水相中并使其吸附到微粒中; 10）聚合单体以在微粒上提供聚合物层; 和11）用一种或多种聚合物，纳米颗粒或生物大分子层官能化微粒的聚合物表面层。 在相关方法中，纳米颗粒设置有亲水表面层，并且通过将水性纳米颗粒分散在连续的疏水相中来制备乳液。 微粒具有聚合物涂层和包含超过颗粒质量的50％的磁芯。 聚合物壳可以含有一个或多个能够形成用于管理各种分离的化学键的官能团。